Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
Purification
ITF1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
ITF1 antibody was raised against a 14 amino acid synthetic peptide near the internal region of ITF1.
ELISA: Antibody detection limit dilution 1:128000. Western Blot: Approximately 75 kDa band observed in lysates of Daudi lysate (calculated MW of 67.6 kDa according to NP_003191.1). Recommended concentration: 0.01-0.03 μg/mL. Immunohistochemistry: In paraffin embedded human kidney shows strong nuclear staining in DCT cells. Recommended concentration, 3-5 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.