This antibody is expected to recognise reported isoform 1 (NP_068779.1) and isoform 3 (NP_001184249.1).
Purification
MCL1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
MCL1 antibody was raised against a 12 amino acid synthetic peptide near the C-Terminus of MCL1.
ELISA: Antibody detection limit dilution 1:2,000. Western Blot: Approximately 35 kDa band observed in lysates of Human Lung, in Mouse and Rat Thymus, and in cell line K562 (calculated MW of 37.3 kDa according to NP_068779.1). Recommended concentration: 1-3 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target
MCL-1 (MCL1)
(Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1))