RICTOR antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
RICTOR antibody was raised against an 11 amino acid synthetic peptide near the C-Terminus of RICTOR.
ELISA: Antibody detection limit dilution 1:4,000. Western Blot: Approximately 38 kDa band in human lung carcinoma A549 cell line and cervix epitheloid carcinoma cell line HeLa lysates after 0.1 μg/mL antibody staining. The 38 kDa band was successfully blocked by incubation with the immunizing peptide.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target
RICTOR
(RPTOR Independent Companion of mTOR, Complex 2 (RICTOR))