This antibody is expected to recognize both reported isoforms (NP_001034750.1 and NP_683708.1).
Purification
TIRAP antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
TIRAP antibody was raised against a 15 amino acid synthetic peptide near the internal region of TIRAP.
ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 24 kDa band observed in lysates of human myeloid erythroblast call line K562 (calculated MW of 23.9 kDa according to NP_001034750.1 and 24.8 kDa according to NP_683708.1). Recommended concentration: 0.1-0.3 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target
TIRAP
(Toll-Interleukin 1 Receptor (TIR) Domain Containing Adaptor Protein (TIRAP))