Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
Purification
DNMT1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
DNMT1 antibody was raised against a 14 amino acid synthetic peptide near the internal egion of DNMT1.
ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 170 kDa band observed in Jurkat lysate (calculated MW of 183 kDa according to NP_001370.1). Recommended concentration: 0.5-1.5 μg/mL. Immunohistochemistry: In paraffin embedded human kidney shows nuclear staining in PCT. Recommended concentration, 3-6 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.