Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Specificity
The antibody detects endogenous levels of ACK1 only when phosphorylated at tyrosine 284.
Purification
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Immunogen
ACK1 (phospho Tyr284) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 284 (D-H-Y (p)-V-M) derived from Human ACK1.
Non-receptor tyrosine-protein and serine/threonine-protein kinase that is implicated in cell spreading and migration, cell survival, cell growth and proliferation. Transduces extracellular signals to cytosolic and nuclear effectors. Phosphorylates AKT1, AR, MCF2, WASL and WWOX. Implicated in trafficking and clathrin-mediated endocytosis through binding to epidermal growth factor receptor (EGFR) and clathrin. Binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR, thereby contributing to the accumulation of EGFR at the limiting membrane of early endosomes.