The antibody detects endogenous levels of AML1 only when phosphorylated at serine 435.
Purification
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Immunogen
AML1 (phospho Ser435) antibody was raised against a peptide sequence around phosphorylation site of Serine 435 ( S-N-S (p)-P-T) derived from Human AML1.
CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. Inhibits MYST4-dependent transcriptional activation.