The antibody detects endogenous levels of Cyclin H only when phosphorylated at threonine 315.
Purification
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Immunogen
Cyclin H (phospho Thr315) antibody was raised against a peptide sequence around phosphorylation site of threonine 315 (E-W-T (p)-D-D) derived from Human Cyclin H .
Regulates CDK7, the catalytic subunit of the CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.