Cyclin H antibody (pThr315)

Catalog No. ABIN2568091

Product Details anti-Cyclin H Antibody

Target: Cyclin H (CCNH)

Binding Specificity: pThr315

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin H antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: The antibody detects endogenous levels of Cyclin H only when phosphorylated at threonine 315.

Purification: Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Immunogen: Cyclin H (phospho Thr315) antibody was raised against a peptide sequence around phosphorylation site of threonine 315 (E-W-T (p)-D-D) derived from Human Cyclin H .

Isotype: IgG

Application Details

Application Notes: Western Blot: 1:500~1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store antibody at -20°C for up to one year.

Target Details for Cyclin H

Target: Cyclin H (CCNH)

Alternative Name: Cyclin H (CCNH Products)

Synonyms: zgc:114132 antibody, cak antibody, p34 antibody, p37 antibody, Cych antibody, ACYPI004637 antibody, ccnh antibody, Cyclin-H antibody, 142767_at antibody, CG7405 antibody, Dmel\\CG7405 antibody, CAK antibody, 6330408H09Rik antibody, AI661354 antibody, AV102684 antibody, AW538719 antibody, ccnh-A antibody, cyclin H antibody, cyclin-H antibody, Cyclin H antibody, CYclin H antibody, cyclin H L homeolog antibody, CCNH antibody, ccnh antibody, LOC5577539 antibody, Cych antibody, CycH antibody, cyh-1 antibody, Ccnh antibody, ccnh.L antibody

Background: Regulates CDK7, the catalytic subunit of the CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.

Molecular Weight: 36 kDa

Gene ID: 902

NCBI Accession: NP_001230

UniProt: P51946

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, M Phase