TCF3 antibody (pThr355)

Catalog No. ABIN2568095

Product Details anti-TCF3 Antibody

Target: TCF3 (Transcription Factor 3 (E2A Immunoglobulin Enhancer Binding Factors E12/E47) (TCF3))

Binding Specificity: pThr355

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TCF3 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Specificity: The antibody detects endogenous levels of E2A only when phosphorylated at threonine 355.

Purification: Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Immunogen: E2A (phospho Thr355) antibody was raised against a peptide sequence around phosphorylation site of threonine 355 (P-S-T (p)-P-V) derived from Human E2A .

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store antibody at -20°C for up to one year.

Target Details for TCF3

Target: TCF3 (Transcription Factor 3 (E2A Immunoglobulin Enhancer Binding Factors E12/E47) (TCF3))

Alternative Name: E2A (TCF3 Products)

Synonyms: E2A antibody, E47 antibody, ITF1 antibody, TCF-3 antibody, VDIR antibody, bHLHb21 antibody, tcf3a antibody, zgc:113045 antibody, A1 antibody, AA408400 antibody, ALF2 antibody, AW209082 antibody, E12 antibody, E12/E47 antibody, ME2 antibody, Pan1 antibody, Pan2 antibody, Tcfe2a antibody, bhlhb21 antibody, e2a antibody, itf1 antibody, tcfe2a antibody, vdir antibody, xtcf-3 antibody, xtcf3 antibody, G12 antibody, TCF7L1 antibody, XE3 antibody, transcription factor 3 antibody, transcription factor 3b antibody, transcription factor 3 L homeolog antibody, transcription factor E2a antibody, TCF3 antibody, tcf3b antibody, Tcf3 antibody, tcf3.L antibody, tcfe2a antibody

Background: E2A is a transcription factor that plays major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Heterodimers between E2A and tissue-specific basic helix-loop-helix (bHLH) Dimers bind DNA on E-box motifs: 5'- CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer. Deletions in E2A have been observed in a subset of pre-B-cell acute lymphoblastic leukemia (B-ALL) cases. Two alternatively spliced human isoforms have been described.

Molecular Weight: 67 kDa

Gene ID: 6929

NCBI Accession: NP_003191

UniProt: P15923

Pathways: WNT Signaling, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Production of Molecular Mediator of Immune Response