The antibody detects endogenous levels of MAP2K3 only when phosphorylated at threonine 222.
Purification
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Immunogen
MAP2K3 (phospho Thr222) antibody was raised against a peptide sequence around phosphorylation site of threonine 222 (A-K-T (p)-M-D) derived from Human MAP2K3 .
The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells.