WEE1 antibody (pSer642)

Catalog No. ABIN2568532

Product Details anti-WEE1 Antibody

Target: WEE1 (WEE1 Homolog (S. Pombe) (WEE1))

Binding Specificity: pSer642

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WEE1 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: The antibody detects endogenous levels of WEE1 only when phosphorylated at serine 642.

Purification: Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Immunogen: WEE1 (phospho Ser642) antibody was raised against a peptide sequence around phosphorylation site of Serine 642 (S-V-S (p)-L-T) derived from Human WEE1.

Isotype: IgG

Application Details

Application Notes: Western Blot: 1:500~1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store antibody at -20°C for up to one year.

Target Details for WEE1

Target: WEE1 (WEE1 Homolog (S. Pombe) (WEE1))

Alternative Name: WEE1 (WEE1 Products)

Synonyms: WEE1A antibody, WEE1hu antibody, ATWEE1 antibody, F22D16.3 antibody, F22D16_3 antibody, WEE1 kinase homolog antibody, WEE1-KINASE antibody, WEE1 antibody, Xe-Wee1B antibody, XeWee1B antibody, wee1-b antibody, wee1a antibody, wee2-a antibody, xe-wee1a antibody, xwee1 antibody, wee1 antibody, wee1b antibody, wee1hu antibody, xwee2 antibody, GB19689 antibody, Wee1A antibody, fa20h03 antibody, wu:fa20h03 antibody, zgc:136514 antibody, WEE1 G2 checkpoint kinase antibody, Wee1 kinase antibody, WEE1-like kinase antibody, WEE1 G2 checkpoint kinase S homeolog antibody, wee1 homolog antibody, WEE1 homolog 2 antibody, wee1-like protein kinase antibody, WEE 1 homolog 1 (S. pombe) antibody, M phase inhibitor protein kinase Wee1 antibody, WEE1 antibody, Wee1 antibody, wee1.S antibody, wee1-a antibody, wee2 antibody, LOC551677 antibody, wee1 antibody

Background: Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.

Molecular Weight: 100 kDa

Gene ID: 7465

NCBI Accession: NP_003381

UniProt: P30291

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, M Phase