VAV2 antibody

Catalog No. ABIN264962

Product Details anti-VAV2 Antibody

Target: VAV2 (Vav 2 Guanine Nucleotide Exchange Factor (VAV2))

Reactivity: Human

Host: Sheep

Clonality: Polyclonal

Conjugate: This VAV2 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Specificity: This antibody reacts to Vav-2 SHD.

Purification: Purified

Isotype: IgG

Application Details

Application Notes: Western Blot: 1: 5,000. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: Phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for VAV2

Target: VAV2 (Vav 2 Guanine Nucleotide Exchange Factor (VAV2))

Alternative Name: VAV2 (VAV2 Products)

Synonyms: VAV-2 antibody, 2810040F13Rik antibody, AI847175 antibody, vav guanine nucleotide exchange factor 2 antibody, vav 2 oncogene antibody, VAV2 antibody, Vav2 antibody

Background: The immunogen used for the anti-human Vav-2 SHD region was a his tagged fusion protein Vav-2 corresponding to amino acid sequence 578 - 878 and having a molecular weight of 100kD. Vav-2 has a molecular weight of 100 kD and shares over a 50 % homology at the amino acid and nucleic acid level with Vav. Vav-2 is a ubiquitously expressed structural homolog of the Vav protooncogene that is expressed preferentially in hematopoetic cells. Both proteins are comprised of a Dbl- homology (DH) domain with guanosine nucleotide exchange (GEF) activity exclusively directed towards Rho/Rac GTPases, a pleckstrin homology (PH) domain, a calponin-homology (CH) region, an acidic domain (AD) a zinc finger butterfly motif, two SH3 regions and one SH2 domain. GEF activity of RhoA family G proteins is induced by tyrosine phosphorylation in wild type vav-2, and is constitutively activated in N terminus deleted oncogene forms. Constitutive expression of a Vav-2 oncoprotein may result in morphological alterations including highly enlarged cells in which karyokinesis and cytokinesis frequently are uncoupled.Synonyms: Guanine nucleotide exchange factor VAV2, VAV-2

Gene ID: 7410

NCBI Accession: NP_001127870

UniProt: P52735

Pathways: Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway