CDC25B antibody

Catalog No. ABIN265341

Product Details anti-CDC25B Antibody

Target: CDC25B (Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC25B antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Specificity: This antibody detects endogenous levels of CDC25B protein (region surrounding Arg317).

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000. IF: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CDC25B

Target: CDC25B (Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B))

Alternative Name: CDC25B (CDC25B Products)

Synonyms: CDC25B antibody, AI604853 antibody, cell division cycle 25B antibody, cdc25b antibody, CDC25B antibody, Cdc25b antibody

Background: The activity of cyclin dependent kinases in cell cycle is regulated by the phosphorylation status, which is controlled by the antagonistic action of wee 1 kinase and Cdc25 phosphatases. Three Cdc25 genes are present in human cells: Cdc25A, Cdc25B and Cdc25C. Cdc25A and Cdc25B are expressed throughout the cell cycle, with peak expression in G1 for Cdc25A and in both G1 S phase and G2 for Cdc25B. Cdc25C is mainly expressed in G2 phase.Synonyms: CDC25HU2, Dual specificity phosphatase Cdc25B, M-phase inducer phosphatase 2

Molecular Weight: approx. 65 kDa

Gene ID: 994

NCBI Accession: NP_004349

UniProt: P30305

Pathways: Cell Division Cycle, M Phase, Autophagy