MEK2 antibody

Catalog No. ABIN265519

Product Details anti-MEK2 Antibody

Target: MEK2 (MAP2K2) (Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MEK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of MEK2 protein. (region surrounding Arg388)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE!

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for MEK2

Target: MEK2 (MAP2K2) (Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2))

Alternative Name: MAPKK 2 (MAP2K2 Products)

Synonyms: CFC4 antibody, MAPKK2 antibody, MEK2 antibody, MKK2 antibody, PRKMK2 antibody, AA589381 antibody, MK2 antibody, Prkmk2 antibody, ATMKK2 antibody, F27B13.50 antibody, F27B13_50 antibody, MAP KINASE KINASE 1 antibody, MAP kinase kinase 2 antibody, MK1 antibody, map2k2 antibody, wu:fa56c06 antibody, zgc:123171 antibody, zgc:172250 antibody, mitogen-activated protein kinase kinase 2 antibody, mitogen activated protein kinase kinase 2 antibody, MAP kinase kinase 2 antibody, mitogen-activated protein kinase kinase 2a antibody, mitogen-activated protein kinase kinase 2b antibody, MAP2K2 antibody, Map2k2 antibody, MKK2 antibody, map2k2a antibody, map2k2b antibody

Background: Activation of extracellular signal-regulated kinase (ERK) or mitogen-activated protein kinase by MEK (mitogen-activated protein kinase or extracellular signal-regulated kinase kinase) is an essential event in the mitogenic growth factor-induced signal transduction pathway. Phosphorylation of MEKs correlates with their ability to phosphorylate and activate ErKs. MEK1 and MEK2 can also be activated by autophosphorylation. Lipopolysaccharide activates many of the MAPK family members of the immediate upstream MAPK activator MEK1, MEK2, and MEK3. In plants, MEK can phosphorylate and activate MAPK, and that Tyr phosphorylation is critical for the catalytic activity of MAPK in plants.Synonyms: Dual specificity mitogen-activated protein kinase kinase 2, ERK activator kinase 2, MAP kinase kinase 2, MAPK/ERK kinase 2, MEK2, MKK2

Molecular Weight: approx. 45 kDa

Gene ID: 5605

NCBI Accession: NP_109587

UniProt: P36507

Pathways: MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling