Rpb1 CTD antibody (pSer2)

Catalog No. ABIN2668545

This Rabbit Polyclonal antibody specifically detects Rpb1 CTD in WB and IP. It exhibits reactivity toward Human.

Quick Overview for Rpb1 CTD antibody (pSer2) (ABIN2668545)

Target: Rpb1 CTD (RNA Polymerase II CTD)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Rpb1 CTD antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-Rpb1 CTD Antibody

Binding Specificity: pSer2

Purpose: RNA pol II CTD phospho Ser2 antibody (pAb)

Purification: Affinity Purified

Immunogen: This RNA pol II CTD phospho Ser2 antibody was raised against a peptide containing the RNA Pol II CTD sequence phosphorylated at serine 2.

Isotype: IgG

Application Details

Application Notes: IP: 5 - 10 µL per IP WB: 1:500 - 1:2,000 dilution For RNA pol II CTD phospho Ser2, we also offer AbFlex RNA pol II CTD phospho Ser2 Recombinant Antibody (rAb). For details, see Catalog No. 91115.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for Rpb1 CTD

Target: Rpb1 CTD (RNA Polymerase II CTD)

Alternative Name: RNA Pol II CTD

Background: RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. During the transcription cycle, the CTD of the large subunit of RNA pol II is reversibly phosphorylated. RNA pol II containing unphosphorylated CTD is recruited to the promoter, whereas the hyperphosphorylated CTD form is involved in active transcription. Phosphorylation occurs at two sites within the heptapeptide repeat, at serine 2, serine 5 and serine 7. RNA pol II phosphorylated at serine 2 is enriched over the gene body and is associated with transcriptional elongation.

Molecular Weight: 240 kDa

Gene ID: 5430

NCBI Accession: NP_000928