SLUG antibody

Catalog No. ABIN2689904

Product Details anti-SLUG Antibody

Target: SLUG (SNAI2) (Snail Family Zinc Finger 2 (SNAI2))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This SLUG antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), BioImaging (BI), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Intracellular Staining (ICS)

Brand: BD Pharmingen™

Characteristics: The S43-1259 monoclonal antibody specifically recognizes SNAI2, also named Slug, which is a zinc finger transcriptional repressor first recognized for its roles in embryogenesis. SNAI2/Slug and other members of the snail family of transcription factors inhibit the expression of E-cadherin, which plays important roles in cell adhesion and in the maintenance of tissue structure. SNAI2/Slug activation and E-cadherin inhibition induces epithelial-mesenchymal transition (EMT) at several stages of embryonic development and also contributes to the invasiveness of malignancies. Aberrant expression of SNAI2/Slug has also been linked to cancer stem cell formation, cell cycle regulation and apoptosis. SNAI2/Slug is a downstream effector of various signaling pathways, including Akt, Wnt and SCF/ckit signaling. Immunoflourescent, flow cytometric, western blot, and immunohistochemical analysis of SNAI2/Slug in human sarcoma cells. FAR LEFT: RH-30 rhabdomyosarcoma cells (DSMZ, ACC 489) were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655), permeabilized with BD Perm/Wash™ Buffer (Cat No. 554723) and stained with Purified Mouse Anti-SNAI2/Slug (pseudo-colored green) at 1.2 μg/mL followed by Alexa Fluor® 488 goat anti-mouse Ig (Life Technologies). Nuclear counter -staining was with DAPI (pseudo-colored blue). The images were captured on a BD Pathway™ 435 Cell Analyzer and merged using BD Attovision™ software. Permeabilization with BD Phosflow™ Perm Buffer III (Cat. No. 558050) or 0.1 % Triton™ X-100 buffer is also suitable. MIDDLE LEFT: RH-30 cells were fixed and permeabilized with the BD Pharmingen™ Transcription Factor Buffer Set (Cat No. 562574) and stained with either Purified Mouse IgG1 κ Isotype Control (Cat No. 554121, dashed line) or Purified Mouse Anti-SNAI2/Slug (solid line), followed by PE Goat Anti-Mouse Ig (Multiple Adsorption) (Cat No. 550589). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry was performed using a BD LSRFortessa™ Cell Analyzer. MIDDLE RIGHT: Lysates of RH-30 cells were electrophoresed (SDS-PAGE), transferred to PVDF membranes and then probed with 125, 60, 30, 15 and 7 ng/mL of Purified Mouse Anti-SNAI2/Slug (lanes 1-5) and HRP goat anti-mouse Ig (Cat. No. 554002). FAR RIGHT: Following antigen retrieval with Retrievagen A Buffer (Cat. No. 550524), formalin-fixed paraffin sections of a human fibrosarcoma were stained with either Purified Mouse IgG1 κ Isotype Control (Cat. No.550878, left) or Purified Mouse Anti-SNAI2/Slug (right). Biotin Goat Anti-Mouse Ig (Multiple Adsorption) (Cat. No. 550337), Streptavidin HRP (Cat. No.550946), and the DAB Substrate Kit (Cat. No. 550880) were used to develop the staining. Original magnification: 40x.

BD Pharmingen™ Purified Mouse Anti-SNAI2/Slug - Purified - Clone S43-1259 - Isotype Mouse IgG1, κ - Reactivity Hu, Ms - 50 μg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human SNAI2 Recombinant Protein

Clone: S43

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-SLUG antibody (ABIN2689904)

Carpenter, Paw, Dewhirst, Lo: "Akt phosphorylates and activates HSF-1 independent of heat shock, leading to Slug overexpression and epithelial-mesenchymal transition (EMT) of HER2-overexpressing breast cancer cells." in: Oncogene, Vol. 34, Issue 5, pp. 546-57, (2015) (PubMed).

Guo, Keckesova, Donaher, Shibue, Tischler, Reinhardt, Itzkovitz, Noske, Zürrer-Härdi, Bell, Tam, Mani, van Oudenaarden, Weinberg: "Slug and Sox9 cooperatively determine the mammary stem cell state." in: Cell, Vol. 148, Issue 5, pp. 1015-28, (2012) (PubMed).

Francí, Takkunen, Dave, Alameda, Gómez, Rodríguez, Escrivà, Montserrat-Sentís, Baró, Garrido, Bonilla, Virtanen, García de Herreros: "Expression of Snail protein in tumor-stroma interface." in: Oncogene, Vol. 25, Issue 37, pp. 5134-44, (2006) (PubMed).

Target Details for SLUG

Target: SLUG (SNAI2) (Snail Family Zinc Finger 2 (SNAI2))

Alternative Name: SNAI2/Slug (SNAI2 Products)

Synonyms: Slug antibody, Slugh antibody, Snail2 antibody, snail family transcriptional repressor 2 antibody, snail family zinc finger 2 antibody, Snai2 antibody

Background: Synonyms: Snai2, SLUG, Slugh, SLUGH

Molecular Weight: 30-34 kDa