Nibrin antibody

Catalog No. ABIN271773

Product Details anti-Nibrin Antibody

Target: Nibrin (NBN)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nibrin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of p95/NBS1 protein. (region surrounding Thr337)

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse.

Purification: Affinity Chromatography using epitope-specific immunogen

Application Details

Application Notes: ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Nibrin

Target: Nibrin (NBN)

Alternative Name: Nibrin (NBN Products)

Synonyms: NBN antibody, AT-V1 antibody, AT-V2 antibody, ATV antibody, NBS antibody, NBS1 antibody, P95 antibody, Nbs1 antibody, im:6911679 antibody, zgc:194152 antibody, nibrin antibody, NBN antibody, nbn antibody, Nbn antibody

Background: DNA repair proteins are necessary for the maintenance of chromosome integrity and are involved in the elimination of premutagenic lesions from DNA. The DNA repair proteins Rad51 and Rad52 are key components of the double-strand-break repair (DSBR) pathway. Rad51 is essential for mitotic and meiotic recombination, and its mutation in yeast and mammalian cells results in chromosome loss. Overexpression of Rad52 confers resistance to ionizing radiation and induces homologous intrachromosomal recombination. Rad52 is thought to be involved in an early stage of Rad51-mediated recombination. Additional proteins involved in the pathway include Dmc1 and nibrin. Dmc1 is specifically involved in meiotic recombination. Nibrin, which complexes with Mre11 and Rad50, is absent in Nijemegen breakage syndrome (NBS) patients.Synonyms: Cell cycle regulatory protein p95, NBN, NBS, NBS1, Nijmegen breakage syndrome protein 1, P95

Molecular Weight: approx. 95 kDa

Gene ID: 4683

NCBI Accession: NP_002476

UniProt: O60934

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response