RAN antibody

Catalog No. ABIN271799

Product Details anti-RAN Antibody

Target: RAN (RAN, Member RAS Oncogene Family (RAN))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAN antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: RAN antibody detects endogenous levels of RAN protein. (region surrounding E202)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: PBS, pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for RAN

Target: RAN (RAN, Member RAS Oncogene Family (RAN))

Alternative Name: RAN (RAN Products)

Synonyms: ARA24 antibody, Gsp1 antibody, TC4 antibody, ran antibody, ara24 antibody, gsp1 antibody, ran-1 antibody, tc4 antibody, RAN antibody, RANP1 antibody, AAF30287 antibody, CG1404 antibody, Dmel\CG1404 antibody, Ran antibody, dran antibody, l(1)G0075 antibody, ran10A antibody, fc16b04 antibody, wu:fc16b04 antibody, RAN, member RAS oncogene family antibody, RAN, member RAS oncogene family S homeolog antibody, CG1404 gene product from transcript CG1404-RC antibody, RAN antibody, Ran antibody, ran.S antibody, ran antibody

Background: The small Ras-related protein Ran, also called TC4, is a 24 kDa, nuclear localized GTPase implicated in a diverse array of cellular processes including DNA replication, entry into and exit from mitosis and the transport of RNA and proteins through the nuclear pore complex. Like Ras, active Ran GTP and inactive Ran GDP levels are tightly regulated by guanine nucleotide exchange factors (GEFs) and GTPase activating proteins (GAPs). The abundant GEF, RCC1 (regulator of chromosome condensation 1), increases the rate at which Ran exchanges GDP for GTP. Ran GAP1 opposes the effects of RCC1 by increasing the rate at which Ran hydrolyzes GTP to GDP. A 23 kDa protein designated Ran BP1 has no intrinsic GAP activity, and functions as a GEF inhibitor deactivating RCC1 and thereby indirectly increasing the ratio of Ran GDP to Ran GTP. The 358 kDa protein Ran BP2 has been proposed as the Ran GTP docking site at the periphery of the nuclear pore complex.Synonyms: ARA24, Androgen receptor-associated protein 24, GTP-binding nuclear protein Ran, GTPase Ran, Ras-like protein TC4, Ras-related nuclear protein

Molecular Weight: approx. 25 kDa

Gene ID: 5901

NCBI Accession: NP_006316

UniProt: P62826

Pathways: Regulatory RNA Pathways, Intracellular Steroid Hormone Receptor Signaling Pathway, Protein targeting to Nucleus