XRCC1 antibody

Catalog No. ABIN271979

Product Details anti-XRCC1 Antibody

Target: XRCC1 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 1 (XRCC1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This XRCC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of XRCC1 protein. (region surrounding Gly552)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity Chromatography using epitope-specific immunogen.

Application Details

Application Notes: ELISA: 1/10000approx. 1/20000. Western Blot: 1/500approx. 1/1000. Immunohistochemistry: 1/50approx. 1: /200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE!

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for XRCC1

Target: XRCC1 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 1 (XRCC1))

Alternative Name: XRCC1 (XRCC1 Products)

Synonyms: xrcc1 antibody, MGC80909 antibody, XRCC1 antibody, rcc antibody, RCC antibody, Xrcc-1 antibody, X-ray repair complementing defective repair in Chinese hamster cells 1 antibody, X-ray repair cross complementing 1 antibody, xrcc1 antibody, XRCC1 antibody, Xrcc1 antibody

Background: The x-ray repair cross-complementing (XRCC) proteins are responsible for efficiently repairing and maintaining genetic stability following DNA base damage. These genes share sequence similarity with the yeast DNA repair protein Rad51. XRCC1 is a 70 kDa protein that facilitates the DNA base excision repair pathway by interacting with DNA ligase III and DNA polymerase to repair DNA single-strand breaks. XRCC2 and XRCC3 are both involved in maintaining chromosome stability during cell division. XRCC2 is required for efficient repair of DNA double-strand breaks by homologous recombination between sister chromatids, and XRCC3 interacts directly with Rad51 to cooperate with Rad51 during recombinational repair. XRCC4 is an accessory factor of DNA ligase IV that preferentially binds DNA with nicks or broken ends. XRCC4 binds to DNA ligase IV and enhances its joining activity, and it is also involved in V(D)J recombination. Any defect in one of the known components of the DNA repair/V(D)J recombination machinery (Ku-70, Ku-80, DNA-PKCS, XRCC4 and DNA ligase IV) leads to abortion of the V(D)J rearrangement process and early block in both T and B cell maturation.Synonyms: DNA repair protein XRCC1, X-ray repair cross-complementing protein 1

Molecular Weight: approx. 90 kDa

Gene ID: 7515

NCBI Accession: NP_006288

UniProt: P18887

Pathways: DNA Damage Repair