LAT antibody

Catalog No. ABIN272032

Product Details anti-LAT Antibody

Target: LAT (Linker For Activation of T Cells (LAT))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LAT antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: This antibody detects endogenous levels of LAT protein. (region surrounding Leu185)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 5000approx. 1: 10000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE!

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for LAT

Target: LAT (Linker For Activation of T Cells (LAT))

Alternative Name: LAT (LAT Products)

Synonyms: LAT antibody, lat antibody, LAT1 antibody, pp36 antibody, linker for activation of T-cells antibody, linker for activation of T cells antibody, LAT antibody, lat antibody, Lat antibody

Background: LAT, a transmembrane adaptor protein expressed in T, NK and mast cells, is an important mediator for T cell receptor (TCR) signaling. Upon TCR engagement, activated Zap-70 phosphorylates LAT at multiple conserved tyrosine residues within SH2 binding motifs, exposing these motifs as the docking sites for downstream signaling targets. The phosphorylation of LAT at Tyr171 and 191 enables the binding of Grb2, Gads/SLP-76, PLCgamma1 and PI3 kinase through their SH2 domain and translocates them to the membrane. This process eventually leads to activation of the corresponding signaling pathways.Synonyms: 36 kDa Phospho-Tyrosine Adapter Protein, Linker For Activation of T-Cells Family Member 1, p36-38, pp36

Molecular Weight: approx. 28 kDa

Gene ID: 27040

NCBI Accession: NP_001014987

UniProt: O43561

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway