PBK antibody

Catalog No. ABIN272056

Product Details anti-PBK Antibody

Target: PBK (PDZ Binding Kinase (PBK))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PBK antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody detects endogenous levels of PBK/TOPK protein. (region surrounding Ile4)

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 21000approx. 1: 5000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should bedetermined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE!

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for PBK

Target: PBK (PDZ Binding Kinase (PBK))

Alternative Name: PBK / TOPK (PBK Products)

Synonyms: PBK antibody, topk antibody, CT84 antibody, Nori-3 antibody, SPK antibody, TOPK antibody, 2810434B10Rik antibody, AW538537 antibody, D14Ertd732e antibody, zgc:92050 antibody, PDZ binding kinase antibody, PDZ binding kinase L homeolog antibody, PBK antibody, pbk antibody, Pbk antibody, pbk.L antibody

Background: PBK (PDZ binding kinase) belongs to the serine/threonine protein kinase family. It is related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family and phosphorylates MAP kinase p38. It is active only in mitosis. When phosphorylated it forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage. It makes a kinase-substrate complex with cdk1/cyclin B1 and PRC1 on microtubules during mitosis, enhances the cdk1/cyclin B1-dependent phosphorylation of PRC1 and thereby strongly promotes cytokinesis. It may play a role in the activation of lymphoid cells.Synonyms: CT84, Cancer/testis antigen 84, Lymphokine-activated killer T-cell-originated protein kinase, MAPKK-like protein kinase, Nori-3, PDZ-binding kinase, SPK, T-LAK cell-originated protein kinase

Molecular Weight: approx. 36 kDa

Gene ID: 55872

NCBI Accession: NP_060962

UniProt: Q96KB5