CTLA4 antibody (FITC)
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- Target See all CTLA4 Antibodies
- CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CTLA4 antibody is conjugated to FITC
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Application
- Flow Cytometry (FACS), ELISA, Blocking Reagent (BR)
- Specificity
- Antibody ANC152.2/8H5 reacts with an extracellular epitope of human CTLA-4 expressed on activated T cells.
- Purification
- Protein A purified
- Immunogen
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Mouse IgG1, kappa.
Type of Immunogen: Purified protein - Clone
- ANC152-2-8H5
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product CTLA4 Primary Antibody
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- Application Notes
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Approved: Block, ELISA, Flo
Usage: Anti-CD152/FITC was tested for binding to CD152-muIg-coated 4u beads in FACS (Inquire). Two x 105 CD152-muIg coated beads per tube were spun down and incubated 45 minutes at 4oC with 80 μL of anti-CD152/FITC at a 1:50 dilution (10 μg/mL). Tubes were washed twice, fixed and analyzed by FACS. Beads stained positive with a mean shift of 2.03 log10 fluorescent units when compared to a Mouse IgG1/FITC Isotype control at a similar concentration. Binding was blocked when beads were pre incubated 10 minutes with 20 μL of 0.5 mg/mL anti-CD152 antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 50 mM sodium phosphate, pH 7.5, 100 mM potassium chloride, 150 mM sodium chloride, 0.2 % BSA, 0.04 % sodium azide, 5 % glycerol
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- avoid freeze thaw cycles
- Storage
- 4 °C
- Storage Comment
- Store at 4°C. Avoid freeze-thaw cycles and prolonged exposure to light.
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- Target
- CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))
- Alternative Name
- CTLA4 / CD152 (CTLA4 Products)
- Synonyms
- CTLA-4 antibody, CTLA4 antibody, CD152 antibody, Cd152 antibody, Ctla-4 antibody, Ly-56 antibody, CD antibody, CELIAC3 antibody, GRD4 antibody, GSE antibody, IDDM12 antibody, sCTLA4 antibody, cytotoxic T-lymphocyte associated protein 4 antibody, cytotoxic T-lymphocyte-associated protein 4 antibody, CTLA4 antibody, Ctla4 antibody
- Background
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Name/Gene ID: CTLA4
Synonyms: CTLA4, CD152, CELIAC3, CD152 antigen, CTLA-4, Celiac disease 3, IDDM12, GRD4, GSE, CD152 isoform - Gene ID
- 1493
- Pathways
- Cancer Immune Checkpoints
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