NGFR antibody

Catalog No. ABIN2743243

Product Details anti-NGFR Antibody

Target: NGFR (Nerve Growth Factor Receptor (NGFR))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NGFR antibody is un-conjugated

Application: Flow Cytometry (FACS), ELISA

Specificity: Antibody ANC271/3D7 binds to recombinant CD271-muIg in EIA, and to endogenous CD271 on cell surface of human Reh cells.

Purification: Protein A purified

Immunogen: Recombinant soluble human CD271.

Type of Immunogen: Recombinant protein

Clone: ANC271-3D7

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA, Flo

Usage: Five x 105 cultured human Reh cells per tube were pelleted and pre incubated 5 minutes with 20 μL of human IgG at 300 μg/mL (to reduce non specific binding) after which they were washed and incubated 45 minutes on ice with 80 μL of anti-CD271 antibody(ANC271/3D7) at 5 μg/mL. Cells were washed twice and incubated with 2o reagent Goat anti-Mouse IgG/FITC after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.76 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 50 mM sodium phosphate,  pH 7.5, 100 mM potassium chloride, 150 mM sodium chloride, 0.5 mg/mL Gentamicin sulfate.

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 4°C, avoid freeze-thaw cycles.

Target Details for NGFR

Target: NGFR (Nerve Growth Factor Receptor (NGFR))

Alternative Name: NGFR / CD271 (NGFR Products)

Synonyms: ngfr antibody, wu:fb38c02 antibody, wu:fc10h01 antibody, CD271 antibody, Gp80-LNGFR antibody, TNFRSF16 antibody, p75(NTR) antibody, p75NTR antibody, LNGFR antibody, RNNGFRR antibody, Tnfrsf16 antibody, p75 antibody, NRH1b antibody, fullback antibody, nrh1 antibody, p75ntr antibody, tnfrsf16 antibody, p75NGFR antibody, nerve growth factor receptor antibody, neurotrophin receptor associated death domain antibody, nerve growth factor receptor S homeolog antibody, nerve growth factor receptor (TNFR superfamily, member 16) antibody, NGFR antibody, nradd antibody, Ngfr antibody, ngfr.S antibody

Background: Name/Gene ID: NGFR
Family: TNF Receptor

Synonyms: NGFR, CD271, CD271 antigen, gp80-LNGFR, Nerve growth factor receptor, p75 ICD, p75(NTR), p75NTR, TNFR superfamily, member 16, NGF receptor, TNFRSF16

Gene ID: 4804

Pathways: NF-kappaB Signaling, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Growth Factor Binding