Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)
Cross-Reactivity
Mouse (Murine)
Cross-Reactivity (Details)
The antibody does not cross react to any other cellular protein
Characteristics
PINK1- selective antibodies were generated against a peptide taken from the C-terminal of the PINK1 protein corresponding to AA from 484-495. The PINK1-selective antibodies are affinity purified on an immobilized antigen based affinity matrix, the isolated antibodies were then stabilized in antibody stabilization buffer for long-term storage. The anti- PINK1-selective antibodies are fully characterized for applications in western blotting and ELISA at the recommended dilutions. The Supplier provides PINK1 Western blot positive control samples in SDS-PAGE sample buffer.
Purification
Affinity Purified
Immunogen
Synthetic peptide corresponding to unique amino acid sequence on human PINK1 protein.
Antibodies were tested in ELISA and western blotting applications at 1:500 dilution using ABIN1686586 samples. Antibody dilutions for these antibodies are for reference only, investigators are expected to determine the optimal conditions. Application of this antibody in other protocols has not yet tested. WB: > 1:500 IMM & IP pull-down assays: n.d. IHC: n.d. Investigators using this antibody in protocols other than listed above can request a complimentary sample of this antibody. n.d. not necessarily means the antibody is not suitable for that application, it simply means we have not yet characterized the antibody for that application. The antibody labels a strong band of PINK1 at 70 kDain ABIN1686586 samples and in several cell lines.
Restrictions
For Research Use only
Format
Liquid
Concentration
0.55-0.75 μg/μL
Storage
-20 °C
Storage Comment
Storage of very dilute antibody solutions is not recommended.
PINK1 (PTEN induced protein kinase 1) is a mitochondrial targeted serine/threonine kinase that promotes cell survival, particularly under conditions of oxidative/metabolic stress. The PINK1 gene encodes for a 70 kDa protein that comprises a highly conserved kinase domain of the Ca2+/calmodulin family. Like most mitochondrial proteins, PINK1 is encoded on the nuclear genome and synthesized as a precursor polypeptide at cytosolic ribosomes. The catalytic domain of PINK1 is not closely related to other protein kinases and it possesses three unique insertions between the beta strands that make up the typical fold of the N-lobe of protein kinases. PINK1 contains a conserved C-terminal non-catalytic region of unknown function. PINK1 protein is cleaved upon entry into the mitochondria to produce two N-terminally truncated protein fragments that localize more to the cytosolic than to the mitochondrial fraction. PINK1 is targeted to its final mitochondrial localization in governed by specific signal sequences and accomplished by chaperone proteins and translocation machinery present in the mitochondrial membranes. The most common mitochondrial targeting signals are cleavable N-terminal extensions, which are initially recognized by the cytosol-exposed receptor proteins of the outer membrane. Missense mutations in PINK1 caused autosomal-recessive inherited Parkinson's disease (PD). The Parkinson disease-associated kinase PINK1 is targeted to mitochondria where it is thought to regulate mitochondrial quality control by promoting the selective autophagic removal of dysfunctional mitochondria.