ID4 antibody (Inhibitor of DNA Binding 4, Dominant Negative Helix-Loop-Helix Protein) (Middle Region)

Details for Product anti-ID4 Antibody No. ABIN2779567, Supplier: Log in to see
Antigen
  • Idb4
  • bHLHb27
  • cb547
  • fb77d04
  • sb:cb547
  • wu:fb77d04
  • zgc:123214
  • bhlhb27
  • id4
  • id4a
  • idb4
  • IDB4
  • inhibitor of DNA binding 4
  • inhibitor of DNA binding 4, HLH protein
  • inhibitor of DNA binding 4, HLH protein S homeolog
  • Id4
  • id4
  • id4.S
  • ID4
Epitope
Middle Region
18
17
9
3
2
2
2
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Zebrafish (Danio rerio)
90
58
41
5
4
2
2
1
Host
Rabbit
70
20
Clonality
Polyclonal
Conjugate
This ID4 antibody is un-conjugated
4
4
4
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
61
43
29
16
13
11
7
1
1
1
Options
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Immunogen The immunogen is a synthetic peptide directed towards the middle region of human ID4
Blocking Peptide Blocking peptide for this product available: ABIN978387
Sequence CYSRLRRLVP TIPPNKKVSK VEILQHVIDY ILDLQLALET HPALLRQPPP
Predicted Reactivity Cow: 85%, Dog: 100%, Guinea Pig: 85%, Horse: 93%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 79%, Rat: 100%, Zebrafish: 86%
Characteristics This is a rabbit polyclonal antibody against ID4. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Plasmids, Primers & others Plasmids, Primers & others ID4 products on genomics-online (e.g. as negative or positive controls)
Alternative Name ID4 (ID4 Antibody Abstract)
Background Transcription factors containing a basic helix-loop-helix (bHLH) motif regulate expression of tissue-specific genes in a number of mammalian and insect systems. DNA-binding activity of the bHLH proteins is dependent on formation of homo- and/or heterodimers. Dominant-negative HLH proteins encoded by Id-related genes, such as ID4, also contain the HLH-dimerization domain but lack the DNA-binding basic domain. Consequently, Id proteins inhibit binding to DNA and transcriptional transactivation by heterodimerization with bHLH proteins.Transcription factors containing a basic helix-loop-helix (bHLH) motif regulate expression of tissue-specific genes in a number of mammalian and insect systems. DNA-binding activity of the bHLH proteins is dependent on formation of homo- and/or heterodimers. Dominant-negative HLH proteins encoded by Id-related genes, such as ID4, also contain the HLH-dimerization domain but lack the DNA-binding basic domain. Consequently, Id proteins inhibit binding to DNA and transcriptional transactivation by heterodimerization with bHLH proteins (Pagliuca et al., 1995 [PubMed 7665172]).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: IDB4, bHLHb27
Protein Interaction Partner: TCF3, TCF4, ID3, MYOD1, HES1,
Protein Size: 161
Molecular Weight 16 kDa
Gene ID 3400
NCBI Accession NM_001546, NP_001537
UniProt P47928
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 161 AA

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-Inhibitor of DNA Binding 4, Dominant Negative Helix-Loop-Helix Protein (ID4) (Middle Region) antibody (ABIN2779567) WB Suggested Anti-ID4 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:62500 Positi...
Western Blotting (WB) image for anti-Inhibitor of DNA Binding 4, Dominant Negative Helix-Loop-Helix Protein (ID4) (Middle Region) antibody (ABIN2779567) Lanes : Lane 1: 10ug MDA-MB231 lysateLane 2: 10ug MCF7 lysate Primary Antibody Dil...
Background publications Russell, Akasaka, Majid, Sugimoto, Loraine Karran, Nagel, Harder, Claviez, Gesk, Moorman, Ross, Mazzullo, Strefford, Siebert, Dyer, Harrison: "t(6;14)(p22;q32): a new recurrent IGH@ translocation involving ID4 in B-cell precursor acute lymphoblastic leukemia (BCP-ALL)." in: Blood, Vol. 111, Issue 1, pp. 387-91, 2007 (PubMed).

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