REST antibody (Middle Region)

Catalog No. ABIN2780696

Product Details anti-REST Antibody

Target: REST (RE1-Silencing Transcription Factor (REST))

Binding Specificity: Middle Region

Reactivity: Human, Rat, Mouse, Cow, Dog, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This REST antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: ANMGMALTND MYDLHELSKA ELAAPQLIML ANVALTGEAS GSCCDYLVGE

Predicted Reactivity: Cow: 86%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 92%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against Rest. It was validated on Western Blot.

Purification: Affinity Purified

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1082 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for REST

Target: REST (RE1-Silencing Transcription Factor (REST))

Alternative Name: Rest (REST Products)

Synonyms: NRSF antibody, XBR antibody, 2610008J04Rik antibody, AA407358 antibody, xbr antibody, nrsf antibody, rest antibody, resta antibody, REST antibody, RE1 silencing transcription factor antibody, RE1-silencing transcription factor antibody, RE1-silencing transcription factor S homeolog antibody, REST antibody, Rest antibody, rest.S antibody, rest antibody, LOC615792 antibody

Background: Rest is a transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Rest restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Rest mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier.
Alias Symbols: 2610008J04Rik, AA407358, MGC150099, NRSF
Protein Interaction Partner: L3mbtl2, Htt, Nanog, Sp3, Ewsr1, Nr0b1, Syt4, Calb1, Scn2a1, Bdnf, Ascl1,
Protein Size: 1082

Molecular Weight: 118 kDa

Gene ID: 19712

NCBI Accession: NM_011263, NP_035393

UniProt: Q8VIG1

Pathways: Negative Regulation of Hormone Secretion, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Chromatin Binding, Positive Regulation of Endopeptidase Activity