DFFB antibody (Middle Region)
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- Target See all DFFB Antibodies
- DFFB (DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase) (DFFB))
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Binding Specificity
- Middle Region
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This DFFB antibody is un-conjugated
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Application
- Western Blotting (WB)
- Sequence
- EYFYGLLFTS ENLKLVHIVC HKKTTHKLNC DPSRIYKPQT RLKRKQPVRK
- Predicted Reactivity
- Human: 100%
- Characteristics
- This is a rabbit polyclonal antibody against DFFB. It was validated on Western Blot using a cell lysate as a positive control.
- Purification
- Affinity Purified
- Immunogen
- The immunogen is a synthetic peptide directed towards the middle region of human DFFB
- Top Product
- Discover our top product DFFB Primary Antibody
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- Application Notes
- Optimal working dilutions should be determined experimentally by the investigator.
- Comment
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Antigen size: 338 AA
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -20 °C
- Storage Comment
- For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
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- Target
- DFFB (DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase) (DFFB))
- Alternative Name
- DFFB (DFFB Products)
- Synonyms
- CAD antibody, CPAN antibody, DFF-40 antibody, DFF2 antibody, DFF40 antibody, LOC100223514 antibody, 40kDa antibody, 5730477D02Rik antibody, Didff antibody, Cad antibody, carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase antibody, DNA fragmentation factor subunit beta antibody, DNA fragmentation factor, beta polypeptide (caspase-activated DNase) antibody, DNA fragmentation factor, beta subunit antibody, CAD antibody, DFFB antibody, dffb antibody, LOC100223514 antibody, Dffb antibody
- Background
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DFFB is a nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. DFFB degrades naked DNA and induces apoptotic morphology.Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene but the biological validity of these variants has not been determined. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: CAD, CPAN, DFF-40, DFF2, DFF40
Protein Interaction Partner: DFFA, UBC, NUBP1, MARCKS, APP, CAND1, COPS5, CUL1, CUL2, CUL5, NEDD8, CIDEB, DFFB, TOP2A,
Protein Size: 338 - Molecular Weight
- 39 kDa
- Gene ID
- 1677
- NCBI Accession
- NM_004402, NP_004393
- UniProt
- O76075
- Pathways
- Apoptosis, Caspase Cascade in Apoptosis
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