PRKAR1A antibody (C-Term)

Catalog No. ABIN2787418

Product Details anti-PRKAR1A Antibody

Target: PRKAR1A (Protein Kinase, CAMP-Dependent, Regulatory, Type I, alpha (Tissue Specific Extinguisher 1) (PRKAR1A))

Binding Specificity: C-Term

Reactivity: Human, Rat, Mouse, Guinea Pig, Sheep, Cow, Rabbit, Horse, Zebrafish (Danio rerio), Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRKAR1A antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: MNRPRAATVV ARGPLKCVKL DRPRFERVLG PCSDILKRNI QQYNSFVSLS

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against PRKAR1A. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human PRKAR1A

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 381 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for PRKAR1A

Target: PRKAR1A (Protein Kinase, CAMP-Dependent, Regulatory, Type I, alpha (Tissue Specific Extinguisher 1) (PRKAR1A))

Alternative Name: PRKAR1A (PRKAR1A Products)

Synonyms: ACRDYS1 antibody, ADOHR antibody, CAR antibody, CNC antibody, CNC1 antibody, PKR1 antibody, PPNAD1 antibody, PRKAR1 antibody, TSE1 antibody, 1300018C22Rik antibody, RIalpha antibody, Tse-1 antibody, Tse1 antibody, RIIA antibody, prkar1a antibody, zgc:92515 antibody, im:7047729 antibody, cnc1 antibody, pkr1 antibody, ppnad1 antibody, prkar1 antibody, tse1 antibody, protein kinase cAMP-dependent type I regulatory subunit alpha antibody, protein kinase, cAMP dependent regulatory, type I, alpha antibody, protein kinase cAMP-dependent type 1 regulatory subunit alpha antibody, protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specific extinguisher 1) a antibody, cAMP-dependent protein kinase type I-alpha regulatory subunit pseudogene antibody, protein kinase cAMP-dependent type I regulatory subunit alpha S homeolog antibody, PRKAR1A antibody, Prkar1a antibody, prkar1aa antibody, LOC707275 antibody, prkar1a.S antibody

Background: CAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. This gene encodes one of the regulatory subunits. This protein was found to be a tissue-specific extinguisher that down-regulates the expression of seven liver genes in hepatoma x fibroblast hybrids. Mutations in this gene cause Carney complex (CNC). This gene can fuse to the RET protooncogene by gene rearrangement and form the thyroid tumor-specific chimeric oncogene known as PTC2. A nonconventional nuclear localization sequence (NLS) has been found for this protein which suggests a role in DNA replication via the protein serving as a nuclear transport protein for the second subunit of the Replication Factor C (RFC40). Three alternatively spliced transcript variants encoding the same protein have been observed.
Alias Symbols: CAR, CNC, CNC1, DKFZp779L0468, MGC17251, PKR1, PPNAD1, PRKAR1, TSE1, ADOHR
Protein Interaction Partner: UBC, RAF1, MAPT, CEP250, GSK3B, AGO3, AGO2, ARPC3, PFDN5, DYRK1B, SET, DYRK1A, PLEKHF2, AKAP10, SOX2, PRKACB, PRKACA, NPM1, CAPN11, RFC2, PPP1CA, SMAD2, HIVEP1, SETD7, PRPF40A, CDK2, CCNE1, SMURF1, Akap7, MEN1, HSP90AA4P, SLC38A5, PRPF31, SAFB, UBD, PATZ1
Protein Size: 381

Molecular Weight: 43 kDa

Gene ID: 5573

NCBI Accession: NM_212471, NP_997636

UniProt: P10644

Pathways: Hedgehog Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Myometrial Relaxation and Contraction, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma