CDK8 antibody (C-Term)

Catalog No. ABIN2792177

Product Details anti-CDK8 Antibody

Target: CDK8 (Cyclin-Dependent Kinase 8 (CDK8))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Zebrafish (Danio rerio), Dog, Rabbit, Horse, Cow, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDK8 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: GLIMTSDYQR SNPHAAYPNP GPSTSQPQSS MGYSATSQQP PQYSHQTHRY

Predicted Reactivity: Cow: 100%, Dog: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against CDK8. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human CDK8

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 464 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for CDK8

Target: CDK8 (Cyclin-Dependent Kinase 8 (CDK8))

Alternative Name: CDK8 (CDK8 Products)

Synonyms: K35 antibody, wu:fk95h11 antibody, cyclin dependent kinase 8 antibody, cyclin-dependent kinase 8 antibody, cyclin-dependent kinase 8 S homeolog antibody, Cyclin-dependent kinase 8 antibody, cyclin dependent kinase 19 antibody, CDK8 antibody, Cdk8 antibody, cdk8 antibody, cdk8.S antibody, cdk-8 antibody, CDK19 antibody

Background: The protein encoded by CDK8 is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase and its regulatory subunit cyclin C are components of the RNA polymerase II holoenzyme complex, which phosphorylates the carboxy-terminal domain (CTD) of the largest subunit of RNA polymerase II. This kinase has also been shown to regulate transcription by targeting the CDK7/cyclin H subunits of the general transcription initiation factor IIH (TFIIH), thus providing a link between the 'Mediator-like' protein complexes and the basal transcription machinery.
Alias Symbols: K35
Protein Interaction Partner: CCNC, MED18, MED15, MED31, MED4, MED13L, MED16, MED6, MED13, MED12, MED24, MED20, MED7, MED27, MED17, MED23, MED21, MED14, HERC2, MED22, MED1, MED11, MED8, MED30, MED10, MED28, MED29, MED9, HNRNPF, CDK8, ERCC3, CCNH, MED19, TCEA1, MBP, CTD, MED26, POLR2A,
Protein Size: 464

Molecular Weight: 53 kDa

Gene ID: 1024

NCBI Accession: NM_001260, NP_001251

UniProt: P49336

Pathways: Cell Division Cycle, Regulation of Lipid Metabolism by PPARalpha