This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen
This IKKb Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y199 of human IKKb.
IKBKB
Reactivity: Human
ELISA, WB, IHC, IF
Host: Rabbit
Monoclonal
3E1
unconjugated
Recombinant Antibody
Application Notes
WB: 1:1000
Restrictions
For Research Use only
Format
Liquid
Buffer
Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date
6 months
Zhu, Moore, Morii, Howard, Culver, Arrendale, Reddy, Evers, Zhang, Sica, Chen, Sun, Fu, Khuri, Shin, Snyder, Shoji: "Synthetic curcumin analog UBS109 inhibits the growth of head and neck squamous cell carcinoma xenografts." in: Current cancer drug targets, Vol. 14, Issue 4, pp. 380-93, (2014) (PubMed).
Target
IKBKB
(Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB))
NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the inhibitory I-kappa-B proteins by kinases (IKKa or IKKb) leads to the dissociation of the inhibitor/NF-kappa-B complex and marks the inhibitors for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs.