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Vimentin antibody

Name: Vimentin antibody
Brand: antibodies-online
SKU: ABIN2854814
Price: 609.18 USD
Availability: InStock

KO Validated VIM Reactivity: Human WB, IHC, ELISA, IF, IHC (p), ICC, IP, IHC (fro) Host: Rabbit Polyclonal unconjugated

WB Image Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody , diluted at 1:10000.

WB Image Vimentin antibody detects Vimentin protein by western blot analysis.

IHC-P Image Vimentin antibody detects Vimentin protein at cytoplasm in rat testis by immunohistochemical analysis.

IHC

IP Image Immunoprecipitation of Vimentin protein from HeLa whole cell extracts using 5 μg of Vimentin antibody, Western blot analysis was performed using Vimentin antibody, EasyBlot anti-Rabbit IgG was used as a secondary reagent.

IHC-P Image Vimentin antibody detects Vimentin protein at cytosol on mouse thymus gland by immunohistochemical analysis.

IHC

IHC-Fr Image Immunofluorescence photomicrographs of frozen sections of mouse brain.

IHC

ICC/IF Image Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using Vimentin, antibody (Green) at 1:500 dilution.

IHC-P Image Immunohistochemical analysis of paraffin-embedded U373 xenograft, using Vimentin, antibody at 1:500 dilution.

IHC

Effects of MCME on migration-related proteins in CL1-0 and CL1-5 cells.

Representative immunoblotting and immunofluorescent analyses for selected differentially expressed proteins identified by proteomic analysis of H9C2 cells in response to doxorubicin treatment and pre-treatment with quercetin.

Fusion progeny exhibit epithelial-mesenchymal transition(A) Representative phase-contrast microscopic images of fusion progeny and parental cells.

FOXF1 mediates reprogramming effects on fusion progeny(A) FOXF1 mRNA levels were determined by real-time PCR in fusion progeny and parent cell lines.

Expression pattern of Notch ligands and receptors in first trimester decidual tissue.

IF (cc)

MEF2B-V5 expression alters the abundance of protein from target genes and increases HEK293A cell migration.

MEF2B mutations alter the abundance of protein from MEF2B target genes and decrease cell migration.

Serum-free suspension culture induces the enrichment of Glioma stem-like cells (GSCs).

Aryl hydrocarbon receptor (AhR) expression in different cells was correlated with E-cadherin and vimentin expression and cell motility.

Level of autophagy-related protein expression in different cells was correlated with their cell motility.

Expression of epithelial-mesenchymal transition (EMT) markers in MCF-7 o/e Snail cells.

Differential expression of epithelial-mesenchymal transition (EMT) markers in MCF-7 o/e Snail cells and MCF-F o/e Snail tissues.

ZEB2-AS1 knockdown inhibits migration, invasion and EMT in HNSCC cells.

Reintroduction of exogenous ZEB2 largely attenuated the phenotypical changes induced by ZEB2-AS1 inhibition.

ZEB2-AS1 is involved for TGF-β1-induced EMT in HNSCC cells.

Lipid loading augments hypoxia mediated secretion of pro-tumorigenic factors.

ISX expression induces TWIST1 and Snail1 expression, and promotes EMTAThe expression levels of ISX, TWIST1, Snail1, fibronectin (FN1), VEGF, and E-cadherin (CDH1) mRNA were examined in a ISX-GFP inducible Tet-ON transformants at 8 h after addition of DOX.

ISX interacts with both BRD4 and PCAF in vitro and in vivoABRD4 peptide (291-314 a.

The expression of ISX, BRD4, and PCAF is correlated with lung cancer metastasisAIHC staining with ISX (brown) or PCAF (brown) antibody in lung tumors from patients with lung cancer.

Sequencing analysis of acetylated recombinant and trypsin-digested wild-type ISX using liquid chromatography-mass spectrometryA, BPCAF acetylation of ISX was detected at lysine residue 69 by liquid chromatography-tandem mass spectrometry (LC-tandem MS-MS).

Cataracts induced epithelial-mesenchymal transition (EMT) progression with significantly elevated N-cadherin and MMP9 expression in DM (+) patients.

IF (cc)

35 images

(17 references)

Catalog No. ABIN2854814

Datasheet as PDF

Target See all Vimentin (VIM) Antibodies

Vimentin (VIM)
Reactivity
332

126

125

61

58

43

42

36

29

21

20

13

13

8

7

6

6

4

3

2

1

1

1

1

1

1

1
Human
Host
189

129

9

6

4

4

2
Rabbit
Compatible Secondaries
Clonality
197

145
Polyclonal
Conjugate
199

19

13

13

11

7

7

7

7

7

7

7

4

4

4

4

4

3

2

2

2

2

2

2

2

1

1
This Vimentin antibody is un-conjugated
Application
281

132

98

97

95

64

48

40

39

27

23

19

8

5

4

4

2

2

2

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity

Cat, Dog, Human, Mouse, Rat

Characteristics

Rabbit Polyclonal antibody to Vimentin (vimentin)
Vimentin antibody

Purification

Purified by antigen-affinity chromatography.

Grade

KO Validated

Immunogen

Recombinant protein encompassing a sequence within the center region of human Vimentin. The exact sequence is proprietary.

Isotype

IgG

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VIM Reactivity: Mammalian WB, FACS, IHC (p), ICC Host: Mouse Monoclonal VI-01 unconjugated

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VIM Reactivity: Human, Mouse WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB41034 unconjugated

anti-Vimentin (VIM) (AA 1-200) antibody
VIM Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

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VIM Reactivity: Human WB, IHC, ICC, IP Host: Mouse Monoclonal C8 unconjugated

anti-Vimentin (VIM) (AA 430-457), (C-Term) antibody
VIM Reactivity: Human WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB16691 unconjugated

anti-Vimentin (VIM) (AA 2-466) antibody
VIM Reactivity: Human, Mouse, Rat WB, IHC, ELISA, FACS Host: Mouse Monoclonal 3A1F2 unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human WB, IHC, StM Host: Rabbit Monoclonal VIM-1937R unconjugated Recombinant Antibody

anti-Vimentin (VIM) antibody
VIM Reactivity: Human, Pig, Cow, Chicken, Dog, Cat, Goat WB, IHC, FACS, StM Host: Mouse Monoclonal VM452 unconjugated

Application Notes

WB: 1:5000-1:50000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment

Positive Control: 293T
Validation: Comparison, KO/KD, Orthogonal, Overexpression

Restrictions

For Research Use only
Format

Liquid

Concentration

0.11 mg/mL

Buffer

1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Navarrete-Bernal, Cervantes-Badillo, Martínez-Herrera, Lara-Torres, Gerson-Cwilich, Zentella-Dehesa, Ibarra-Sánchez, Esparza-López, Montesinos, Cortés-Morales, Osorio-Pérez, Villegas-Osorno et al.: "Biological Landscape of Triple Negative Breast Cancers Expressing CTLA-4. ..." in: Frontiers in oncology, Vol. 10, pp. 1206, (2020) (PubMed).

Klein, Lohr, Droste: "Age-Dependent Heterogeneity of Murine Olfactory Bulb Astrocytes." in: Frontiers in aging neuroscience, Vol. 12, pp. 172, (2020) (PubMed).

Horn, Riskin, Hempel, Fousek, Lind, Hamilton, McCampbell, Maeda, Zebala, Su, Schlom, Palena: "Simultaneous inhibition of CXCR1/2, TGF-β, and PD-L1 remodels the tumor and its microenvironment to drive antitumor immunity." in: Journal for immunotherapy of cancer, Vol. 8, Issue 1, (2020) (PubMed).

Wang, Liu, Jeng, Chiang, Chai, Chiou, Huang, Yokoyama, Wang, Huang, Hsu: "PCAF-mediated acetylation of ISX recruits BRD4 to promote epithelial-mesenchymal transition." in: EMBO reports, Vol. 21, Issue 2, pp. e48795, (2020) (PubMed).

Diao, Ge, Song, Wu, Li, Li, Yang, Wang, Cheng: "Overexpression of ZEB2-AS1 promotes epithelial-to-mesenchymal transition and metastasis by stabilizing ZEB2 mRNA in head neck squamous cell carcinoma." in: Journal of cellular and molecular medicine, Vol. 23, Issue 6, pp. 4269-4280, (2020) (PubMed).

Xie, Zhao, Guo, Liu, Fu, Fan, Lin, Chen, Wang, Fan, Wei: "Benzyl isothiocyanate suppresses development and metastasis of murine mammary carcinoma by regulating the Wnt/β‑catenin pathway." in: Molecular medicine reports, Vol. 20, Issue 2, pp. 1808-1818, (2019) (PubMed).

Offer, Menard, Pérez, de Oliveira, Chandran, Johansson, Bång-Rudenstam, Siesjö, Ebbesson, Hedenfalk, Sundgren, Darabi, Belting: "Extracellular lipid loading augments hypoxic paracrine signaling and promotes glioma angiogenesis and macrophage infiltration." in: Journal of experimental & clinical cancer research : CR, Vol. 38, Issue 1, pp. 241, (2019) (PubMed).

Hua, Liu, Hsu, Cheng, Yang, Chang, Lee, Hsiao, Juan, Chien, Yang: "3'UTR polymorphisms of carbonic anhydrase IX determine the miR-34a targeting efficiency and prognosis of hepatocellular carcinoma." in: Scientific reports, Vol. 7, Issue 1, pp. 4466, (2019) (PubMed).

Chu, Lee, Lee, Wang, Huang, Lan, Lin, Lin, Tian, Liu, Chow: "MiR-338-5p promotes metastasis of colorectal cancer by inhibition of phosphatidylinositol 3-kinase, catalytic subunit type 3-mediated autophagy pathway." in: EBioMedicine, Vol. 43, pp. 270-281, (2019) (PubMed).

Nakazawa, Taniyama, Sanada, Morishita, Nakamori, Morimoto, Yeung, Yang: "Periostin blockade overcomes chemoresistance via restricting the expansion of mesenchymal tumor subpopulations in breast cancer." in: Scientific reports, Vol. 8, Issue 1, pp. 4013, (2019) (PubMed).

Chiu, Wen, Wang, Hsu, Tsai, Hung, Tseng, Shyr: "Role of estrogen receptors and Src signaling in mechanisms of bone metastasis by estrogen receptor positive breast cancers." in: Journal of translational medicine, Vol. 15, Issue 1, pp. 97, (2018) (PubMed).

Wang, Wu, Yeh, Su, Hsia, Liang, Chen, Hsueh, Chen: "Melatonin suppresses hepatocellular carcinoma progression via lncRNA-CPS1-IT-mediated HIF-1α inactivation." in: Oncotarget, Vol. 8, Issue 47, pp. 82280-82293, (2017) (PubMed).

Hsu, Chang, Hsu, Liu, Yeh, Wang, Liou, Ko, Chang, Chuang: "Suberoylanilide hydroxamic acid represses glioma stem-like cells." in: Journal of biomedical science, Vol. 23, Issue 1, pp. 81, (2017) (PubMed).

Yin, Yin, Wang, Zhou, Liu, Yang, Wang, Lu: "MiR-206 suppresses epithelial mesenchymal transition by targeting TGF-β signaling in estrogen receptor positive breast cancer cells." in: Oncotarget, Vol. 7, Issue 17, pp. 24537-48, (2017) (PubMed).

Marquez-Vilendrer, Rai, Gramling, Lu, Reisman: "Loss of the SWI/SNF ATPase subunits BRM and BRG1 drives lung cancer development." in: Oncoscience, Vol. 3, Issue 11-12, pp. 322-336, (2016) (PubMed).

Hou, You, Yang, Pan, Chen, Lee, Lin, Liou, Liu, Chi, Ger, Tsai: "Aberrant DNA hypomethylation of miR-196b contributes to migration and invasion of oral cancer." in: Oncology letters, Vol. 11, Issue 6, pp. 4013-4021, (2016) (PubMed).

Wei, Nickoloff, Chen, Liu, Lo, Chang, Yang, Wu, Williams, Gelovani, Deng: "FOXF1 mediates mesenchymal stem cell fusion-induced reprogramming of lung cancer cells." in: Oncotarget, Vol. 5, Issue 19, pp. 9514-29, (2015) (PubMed).
Target

Vimentin (VIM)

Alternative Name

vimentin (VIM Products)

Synonyms

CTRCT30 antibody, cb28 antibody, vime antibody, vim antibody, vim1 antibody, vim2 antibody, VIM antibody, Vimentin antibody, vim4 antibody, vimentin antibody, vimentin L homeolog antibody, vimentin S homeolog antibody, VIM antibody, Vim antibody, vim antibody, vim.L antibody, vim.S antibody

Background

Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.[supplied by OMIM]

Molecular Weight

54 kDa

Gene ID

7431

UniProt

P08670

Pathways

Caspase Cascade in Apoptosis