CDK6 antibody

Catalog No. ABIN2855743

Product Details anti-CDK6 Antibody

Target: CDK6 (Cyclin-Dependent Kinase 6 (CDK6))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDK6 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Rabbit Polyclonal antibody to CDK6 (cyclin-dependent kinase 6)
CDK6 antibody [N1C3]

Purification: Purified by antigen-affinity chromatography.

Grade: KO Validated

Immunogen: Recombinant protein encompassing a sequence within the center region of human CDK6. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Validatio: KO/KD, Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.4 mg/mL

Buffer: 1XPBS pH 7, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-CDK6 antibody (ABIN2855743)

Méndez-Barbero, Gutierrez-Muñoz, Madrigal-Matute, Mínguez, Egido, Michel, Martín-Ventura, Esteban, Blanco-Colio: "A major role of TWEAK/Fn14 axis as a therapeutic target for post-angioplasty restenosis." in: EBioMedicine, Vol. 46, pp. 274-289, (2020) (PubMed).

Zhang, Liu, Li, Cao, Wan, Xi, Li, Tan, Wang, Wu, Ma, Gao: "Prognostic and therapeutic value of disruptor of telomeric silencing-1-like (DOT1L) expression in patients with ovarian cancer." in: Journal of hematology & oncology, Vol. 10, Issue 1, pp. 29, (2017) (PubMed).

Target Details for CDK6

Target: CDK6 (Cyclin-Dependent Kinase 6 (CDK6))

Alternative Name: cyclin dependent kinase 6 (CDK6 Products)

Synonyms: CDK6 antibody, PLSTIRE antibody, 5830411I20 antibody, AI504062 antibody, Crk2 antibody, cyclin dependent kinase 6 antibody, cyclin-dependent kinase 6 antibody, CDK6 antibody, Cdk6 antibody

Background: The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase, which is controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb.

Molecular Weight: 37 kDa

Gene ID: 1021

UniProt: Q00534

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases