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GFP antibody (HRP)

GFP Reactivity: Aequorea victoria WB, ELISA, IHC Host: Goat Polyclonal HRP

Catalog No. ABIN288233

Datasheet as PDF

Target See all GFP Antibodies

GFP (Green Fluorescent Protein (GFP))
Reactivity
175

19

15

15

12

7

5

4

2

1

1

1

1
Aequorea victoria
Host
74

73

35

11

9

6

2

1
Goat
Compatible Secondaries
Clonality
121

85
Polyclonal
Conjugate
104

14

12

9

6

4

4

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This GFP antibody is conjugated to HRP
Application
173

87

55

42

33

31

29

21

16

14

14

14

12

6

4

4

3

3

2

1

1

1

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Cross-Reactivity (Details)

Minimum cross reactivity to Human, Mouse and Rat serum proteins1 mg/ml

Purification

Affinity chromatography purified

Immunogen

GFP antibody (HRP) was raised in goat using a GFP fusion protein corresponding to the full length amino acid sequence (246 aa) derived from the jellyfish Aequorea victoria as the immunogen.

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anti-Green Fluorescent Protein (GFP) (AA 246) antibody
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anti-Green Fluorescent Protein (GFP) antibody
GFP Reactivity: Aequorea victoria WB, ELISA, IP, IC, ChIP-seq Host: Rat Monoclonal 1A5 unconjugated

anti-Green Fluorescent Protein (GFP) antibody
GFP Reactivity: Aequorea victoria WB, ELISA, IP Host: Rabbit Polyclonal unconjugated

anti-Green Fluorescent Protein (GFP) antibody
GFP Reactivity: Aequorea victoria WB, ELISA, IHC, FACS Host: Mouse Monoclonal 4B10B2 unconjugated

anti-Green Fluorescent Protein (GFP) (AA 246) antibody
GFP Reactivity: Aequorea victoria WB, ELISA, FM Host: Rabbit Polyclonal unconjugated

anti-Green Fluorescent Protein (GFP) antibody
GFP Reactivity: Aequorea victoria WB, IF, IHC (fro), IHC (p), IEM Host: Goat Polyclonal unconjugated

anti-Green Fluorescent Protein (GFP) (AA 246) antibody (FITC)
GFP Reactivity: Aequorea victoria FACS, IM Host: Goat Polyclonal FITC

Application Notes

ELISA: 1:10,000-1:50,000, IHC: 1:500-1:2,000, WB: 1:2,000 - 1:5,000
Optimal conditions should be determined by the investigator.

Restrictions

For Research Use only
Format

Lyophilized

Concentration

Lot specific

Buffer

Lyophilized from 0.02 M K2 O4, pH 7.2, with 0.12 NaCl, 10 mg/mL BSA, and 0.01 % gentamycin sulfate. DO NOT add NaN3. Immunoglobulin and protease free.

Preservative

Without preservative

Handling Advice

Avoid repeated freeze/thaw cycles.
Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Dilute only prior to immediate use.

Storage

4 °C/-20 °C

Storage Comment

Store at 4 °C for short term storage. Aliquot and store at -20 °C for long term storage.
Target

GFP (Green Fluorescent Protein (GFP))

Alternative Name

GFP (GFP Products)

Synonyms

green fluorescent protein antibody, gfp antibody

Target Type

Viral Protein

Background

Green fluorescent protein is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining.