Phosphothreonine antibody

Catalog No. ABIN289524

Product Details anti-Phosphothreonine Antibody

Target: Phosphothreonine

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Phosphothreonine antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC)

Cross-Reactivity (Details): Recognizes peptide and proteins phosphorylated on threonine residues. Not cross-reacted with phosphotyrosine.0.05% NaN3

Purification: Purified

Immunogen: Phosphothreonine antibody was raised in rabbit using phosphothreonine-KLH and phosvitin mixture as the immunogen.

Application Details

Application Notes: Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: Lot specific

Buffer: PBS, with 0.05 % NaN3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: -20 °C

Storage Comment: Aliquot and store at -20 °C.

References for anti-Phosphothreonine antibody (ABIN289524)

Mandal, Sharma, Kruse, Sander-Juelch, Munro, Wang, Vilg, Tamás, Bhattacharjee, Wiese, Mukhopadhyay: "Modulation of Leishmania major aquaglyceroporin activity by a mitogen-activated protein kinase." in: Molecular microbiology, Vol. 85, Issue 6, pp. 1204-18, (2013) (PubMed).

Target Details for Phosphothreonine

Target: Phosphothreonine

Abstract: Phosphothreonine Products

Target Type: Amino Acid

Background: Threonine is one of the 20 naturally occurring amino acids. Kinases phosphorylate threonine to phosphothreonine which enters various biosynthetic pathways in the body. The threonine residue is susceptible to numerous posttranslational modifications. The hydroxy side-chain can undergo O-linked glycosylation. In addition, threonine residues undergo phosphorylation through the action of a threonine kinase. In its phosphorylated form, it can be referred to as phosphothreonine.