RBPJ antibody (AA 208-500)

Catalog No. ABIN295799

Product Details anti-RBPJ Antibody

Target: RBPJ (Recombination Signal Binding Protein For Immunoglobulin kappa J Region (RBPJ))

Binding Specificity: AA 208-500

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This RBPJ antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Specificity: Selected for its ability to recognize human CSL in the applications listed.

Purification: Affinity purified

Immunogen: E. coli-derived rhCSL (aa208-500).

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, ICC, WB

Usage: Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect human CSL. The detection limit for rhCSL is approximately 10 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: This antibody can be used at a concentration of 10 μg/mL to detect human CSL on HASMC (human aortic smooth muscle) cells. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by NorthernLights 557-coupled donkey anti-goat IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human CSL. The detection limit for rhCSL is approximately 5 ng/well.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: deionized water

Concentration: Lot specific

Buffer: Lyophilized.

Target Details for RBPJ

Target: RBPJ (Recombination Signal Binding Protein For Immunoglobulin kappa J Region (RBPJ))

Alternative Name: RBPJ (RBPJ Products)

Synonyms: AOS3 antibody, CBF1 antibody, IGKJRB antibody, IGKJRB1 antibody, KBF2 antibody, RBP-J antibody, RBPJK antibody, RBPSUH antibody, SUH antibody, csl antibody, AI843960 antibody, Igkjrb antibody, Igkrsbp antibody, RBP-J kappa antibody, RBPjk antibody, Rbpsuh antibody, Su(H) antibody, XSu(H) antibody, xsu(h)1 antibody, Su(H)1 antibody, Su(h)A antibody, ik:tdsubc_1e8 antibody, rbpj antibody, rbpsuh antibody, xx:tdsubc_1e8 antibody, Su(H)2 antibody, su(h)B antibody, recombination signal binding protein for immunoglobulin kappa J region antibody, recombination signal binding protein for immunoglobulin kappa J region S homeolog antibody, recombination signal binding protein for immunoglobulin kappa J region a antibody, recombination signal binding protein for immunoglobulin kappa J region b antibody, RBPJ antibody, Rbpj antibody, rbpj.S antibody, rbpja antibody, rbpjb antibody

Background: Name/Gene ID: RBPJ

Synonyms: RBPJ, AOS3, CBF1, Csl, KBF2, H-2K binding factor-2, RBP-JK, SUH, Suppressor of hairless homolog, RBP-J, RBP-J kappa, RBPJK, RBPSUH, CBF-1, IGKJRB, IGKJRB1

Gene ID: 3516

UniProt: Q06330

Pathways: Notch Signaling, Stem Cell Maintenance, Smooth Muscle Cell Migration