LEF1 antibody (Lymphoid Enhancer-Binding Factor 1) (AA 257-270)

Details for Product anti-LEF1 Antibody No. ABIN297259, Supplier: Log in to see
Antigen
  • LEF1
  • 3000002B05
  • AI451430
  • Lef-1
  • LEF-1
  • TCF10
  • TCF1ALPHA
  • TCF7L3
  • lymphoid enhancer binding factor 1
  • lymphoid enhancer-binding factor 1
  • lymphoid enhancer factor XLEF-1B
  • LEF1
  • lef1
  • Lef1
Alternatives
anti-Human LEF1 antibody for Enzyme Immunoassay
Epitope
AA 257-270
15
13
13
9
8
6
6
6
4
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Bat, Chicken, Cow (Bovine), Dog (Canine), Hamster, Horse (Equine), Human, Monkey, Mouse (Murine), Pig (Porcine), Rat (Rattus), Zebrafish (Danio rerio)
127
39
29
10
7
6
4
4
4
4
3
3
2
1
1
Host
Goat
56
52
15
5
Clonality
Polyclonal
Conjugate
This LEF1 antibody is un-conjugated
3
3
3
3
3
3
Application
ELISA, Western Blotting (WB)
117
67
23
18
9
7
4
3
2
2
2
1
1
1
1
Options
Supplier
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Immunogen Synthetic peptide C-QHEQRKEQEPKRPH from an internal region of human LEF1 (NP_057353.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Pig, Opossum, Turkey, Chicken, Platypus, Stickleback, Zebrafish (100%), Xenopus (93%), Pufferfish (86%).

Type of Immunogen: Synthetic peptide
Specificity Human LEF1.
Predicted Reactivity Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Pig, Opossum, Turkey, Chicken, Platypus, Stickleback, Zebrafish (100%) Xenopus (93%) Pufferfish (86%).
Purification Immunoaffinity purified
Alternative Name LEF1 (LEF1 Antibody Abstract)
Background Name/Gene ID: LEF1

Synonyms: LEF1, LEF-1, TCF7L3, TCF1-alpha, TCF10, TCF1ALPHA
Gene ID 51176
NCBI Accession NP_057353
UniProt Q9UJU2
Pathways WNT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Hormone Metabolic Process, Nuclear Hormone Receptor Binding, Chromatin Binding
Application Notes Approved: ELISA (1:32000), WB (1 - 3 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 48 kDa band observed in lysates of cell lines Daudi and Jurkat (calculated MW of 44.2 kDa according to NP_057353.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Yasumoto et al , EMBO J. 2002 Jun 3,21(11):2703-14., PMID: 12032083). Recommended concentration: 1-3 μg/mL.

Not recommended for: IHC-P
Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer Tris-buffered saline, pH 7.3, 0.5 % BSA, 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Store at -20°C. Minimize freezing and thawing.
Supplier Images
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