LEF1 antibody (AA 257-270)
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- Target See all LEF1 Antibodies
- LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))
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Binding Specificity
- AA 257-270
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Reactivity
- Human, Mouse, Rat, Dog, Cow, Horse, Pig, Zebrafish (Danio rerio), Monkey, Chicken, Bat, Hamster
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This LEF1 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA
- Specificity
- Human LEF1.
- Predicted Reactivity
- Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Pig, Opossum, Turkey, Chicken, Platypus, Stickleback, Zebrafish (100%) Xenopus (93%) Pufferfish (86%).
- Purification
- Immunoaffinity purified
- Immunogen
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Synthetic peptide C-QHEQRKEQEPKRPH from an internal region of human LEF1 (NP_057353.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Pig, Opossum, Turkey, Chicken, Platypus, Stickleback, Zebrafish (100%), Xenopus (93%), Pufferfish (86%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product LEF1 Primary Antibody
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- Application Notes
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Approved: Peptide-ELISA (1:32000), WB (1 - 3 μg/mL)
Usage: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 48 kDa band observed in lysates of cell lines Daudi and Jurkat (calculated MW of 44.2 kDa according to NP_057353.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Yasumoto et al , EMBO J. 2002 Jun 3,21(11):2703-14., PMID: 12032083). Recommended concentration: 1-3 μg/mL.
Not recommended for: IHC-P - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Tris-buffered saline, pH 7.3, 0.5 % BSA, 0.02 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Store at -20°C. Avoid freeze-thaw cycles.
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- Target
- LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))
- Alternative Name
- LEF1 (LEF1 Products)
- Synonyms
- LEF1 antibody, 3000002B05 antibody, AI451430 antibody, Lef-1 antibody, LEF-1 antibody, TCF10 antibody, TCF1ALPHA antibody, TCF7L3 antibody, lymphoid enhancer binding factor 1 antibody, lymphoid enhancer-binding factor 1 antibody, lymphoid enhancer factor XLEF-1B antibody, LEF1 antibody, lef1 antibody, Lef1 antibody
- Background
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Name/Gene ID: LEF1
Synonyms: LEF1, LEF-1, TCF7L3, TCF1-alpha, TCF10, TCF1ALPHA - Gene ID
- 51176
- NCBI Accession
- NP_057353
- UniProt
- Q9UJU2
- Pathways
- WNT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Hormone Metabolic Process, Nuclear Hormone Receptor Binding, Chromatin Binding
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