Fibronectin 1 antibody (Biotin)
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- Target See all Fibronectin 1 (FN1) Antibodies
- Fibronectin 1 (FN1)
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Reactivity
- Human, Mouse, Rat, Cow, Monkey
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Fibronectin 1 antibody is conjugated to Biotin
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
- Specificity
- Other extracellular matrix proteins was detected by ELISA against purified standards. This antibody reacts with human Fibronectin and has negligible cross- reactivity with Type I, II, III, IV, V or VI Collagens or Laminin. Non-specific cross-reaction of anti-Fibronectin antibodies with other human serum proteins or non-Fibronectin extracellular matrix proteins is negligible
- Purification
- Immunoaffinity purified
- Immunogen
- Fibronectin was purified from Human plasma by binding to a denatured gelatin column followed by elution with high concentrations of arginine. The eluted material was further purified by gel filtration. Immunization occurred after single- band purity was assessed by SDS-PAGE.
- Top Product
- Discover our top product FN1 Primary Antibody
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- Application Notes
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Approved: ELISA (1:4000 - 1:8000), IHC, IHC-Fr (1:50 - 1:200), IP, WB (1:10000)
Usage: Anti-Fibronectin antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for western blotting for highly sensitive qualitative analysis. This product was assayed by immunoblot and found to be reactive against Fibronectin at a dilution of 1:5000 to 1:10000. This product was also assayed against 1.0 μg of Fibronectin in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS as a substrate for 30 minutes at room temperature. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- deionized water
- Concentration
- Lot specific
- Buffer
- Lyophilized, 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 10 mg/mL BSA (IgG, protease free), 0.01 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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- Target
- Fibronectin 1 (FN1)
- Alternative Name
- FN1 / Fibronectin (FN1 Products)
- Synonyms
- FN1 antibody, FN antibody, cig antibody, fibronectin antibody, finc antibody, lets antibody, msf antibody, Fn antibody, fn2 antibody, fb80d10 antibody, wu:fb80d10 antibody, CIG antibody, ED-B antibody, FINC antibody, FNZ antibody, GFND antibody, GFND2 antibody, LETS antibody, MSF antibody, E330027I09 antibody, Fn-1 antibody, FIBNEC antibody, fn-1 antibody, fibronectin 1 antibody, fibronectin 1a antibody, fibronectin 1 S homeolog antibody, FN1 antibody, fn1 antibody, fn1a antibody, Fn1 antibody, fn1.S antibody
- Background
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Name/Gene ID: FN1
Synonyms: FN1, CIG, Cold-insoluble globulin, Fibronectin 1, FINC, LETS, GFND, ED-B, Fibronectin, FN, FNZ, GFND2, Migration-stimulating factor, MSF - Gene ID
- 2335
- UniProt
- P02751
- Pathways
- Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy
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