His Tag antibody

Catalog No. ABIN3020556

Product Details anti-His Tag Antibody

Target: His Tag

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This His Tag antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)

Sequence: HHHHHH

Purification: Affinity purification

Immunogen: A 6×His synthetic peptide (KLH-coupled).

Isotype: IgG

Application Details

Application Notes: WB: 1:5000, IF: 1:2000, IP: 1:100

Restrictions: For Research Use only

Handling

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C / -80 °C

References for anti-His Tag antibody (ABIN3020556)

Yang, Gu, Cheung, Yan, Tong, Jiang, Yang: "InsP3R-SEC5 interaction on phagosomes modulates innate immunity to Candida albicans by promoting cytosolic Ca2+ elevation and TBK1 activity." in: BMC biology, Vol. 16, Issue 1, pp. 46, (2018) (PubMed).

Zhu, Yuan, Fan, Fan, Yang, Yin, Liu, Liu, Cao, Tian, Xue: "The synchronous TAG production with the growth by the expression of chloroplast transit peptide-fused ScPDAT in Chlamydomonas reinhardtii." in: Biotechnology for biofuels, Vol. 11, pp. 156, (2018) (PubMed).

Liu, Xu, Zhu, Zhang, Chen, Zhou, Chen, Lin: "The calcium-dependent kinase OsCPK24 functions in cold stress responses in rice." in: Journal of integrative plant biology, Vol. 60, Issue 2, pp. 173-188, (2018) (PubMed).

Liu, Li, Xie, Jia, Hou, Zou, Zhong: "Enhanced bacterial cellulose production by Gluconacetobacter xylinus via expression of Vitreoscilla hemoglobin and oxygen tension regulation." in: Applied microbiology and biotechnology, Vol. 102, Issue 3, pp. 1155-1165, (2018) (PubMed).

Zhou, Zhao, Ni, Su, Wang, Xu: "Effects of environmental factors on C-type lectin recognition to zooxanthellae in the stony coral Pocillopora damicornis." in: Fish & shellfish immunology, Vol. 79, pp. 228-233, (2018) (PubMed).

Jia, Wang, Zhang, Wang, Lv, Wang, Song: "Identification of a clip domain serine proteinase involved in immune defense in Chinese mitten crab Eriocheir sinensis." in: Fish & shellfish immunology, Vol. 74, pp. 332-340, (2018) (PubMed).

Tao, Shi, Tang, Duszynski, Wang, Li, Suo, Tian, Liu, Suo et al.: "Transgenic Eimeria magna Pérard, 1925 Displays Similar Parasitological Properties to the Wild-type Strain and Induces an Exogenous Protein-Specific Immune Response in Rabbits (Oryctolagus cuniculus ..." in: Frontiers in immunology, Vol. 8, pp. 2, (2017) (PubMed).

Wang, Li, Lu, Hu, Lin, Zeng, Zhou, Liu: "Molecular Characterization, Tissue Distribution and Expression, and Potential Antiviral Effects of TRIM32 in the Common Carp (Cyprinus carpio)." in: International journal of molecular sciences, Vol. 17, Issue 10, (2017) (PubMed).

Jia, Zhang, Jiang, Wang, Wang, Song: "Comparative study of two single CRD C-type lectins, CgCLec-4 and CgCLec-5, from pacific oyster Crassostrea gigas." in: Fish & shellfish immunology, Vol. 59, pp. 220-232, (2017) (PubMed).

Wang, Kuang, Lu, Lin, Liu: "Characterization and biological function analysis of the TRIM47 gene from common carp (Cyprinus carpio)." in: Gene, Vol. 627, pp. 188-193, (2017) (PubMed).

Wang, Li, Xue, Cheng, Du: "Construction of a series of pCS2+ backbone-based Gateway vectors for overexpressing various tagged proteins in vertebrates." in: Acta biochimica et biophysica Sinica, Vol. 48, Issue 12, pp. 1128-1134, (2017) (PubMed).

Zhang, Wang, Zhong, Luo, Shang, Liu, Chen, Fang, Xiao: "Ubiquitin-specific Protease 15 Negatively Regulates Virus-induced Type I Interferon Signaling via Catalytically-dependent and -independent Mechanisms." in: Scientific reports, Vol. 5, pp. 11220, (2016) (PubMed).

Xu, Liu, Cai, Zhang, Xiong: "Heterologous expression and comparative characterization of vacuolar invertases from Cu-tolerant and non-tolerant populations of Elsholtzia haichowensis." in: Plant cell reports, Vol. 34, Issue 10, pp. 1781-90, (2016) (PubMed).

Yan, Tian, Wang, Shao, Zheng, Zhang, Guo, Ding: "Pollen developmental defects in ZD-CMS rice line explored by cytological, molecular and proteomic approaches." in: Journal of proteomics, Vol. 108, pp. 110-23, (2015) (PubMed).

Zhang, Li, Peng, Tang, Zhu, Qian, Xu, Qian: "Glycoprotein E2 of classical swine fever virus expressed by baculovirus induces the protective immune responses in rabbits." in: Vaccine, Vol. 32, Issue 49, pp. 6607-13, (2015) (PubMed).

Target Details for His Tag

Target: His Tag

Alternative Name: His-Tag (His Tag Products)

Target Type: Tag

Background: Plasmid vectors for the expression of coding regions of eukaryotic genes in bacterial, insect and mammalian hosts are in common usage, such expression vectors are frequently used to encode hybrid fusion proteins consisting of a eukaryotic target protein and a specialized region designed to aid in the purification and visualization of the target protein. A system that has proven to be very successful relies on the insertion of a six histidine (His6) sequence in the N-terminus of the encoded protein, allowing for efficient coupling to Ni++- chelating resins and purification by single step affinity chromatography. This polyhistidine sequence can then be removed by specific cleavage at sites recognized by enzymes such as thrombin or enterokinase, permitting the separation of the target protein from the polyhistidine tag. Visualization of such fusion proteins can be achieved by utilizing antibodies generated against specific peptide sequences downstream from the multiple cloning site.