Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

His Tag antibody

WB, IP, IF Host: Mouse Monoclonal unconjugated
Catalog No. ABIN3020556
  • Target See all His Tag Antibodies
    His Tag
    Reactivity
    Please inquire
    Host
    • 85
    • 53
    • 11
    • 6
    • 5
    • 4
    • 1
    • 1
    Mouse
    Clonality
    • 86
    • 67
    • 10
    Monoclonal
    Conjugate
    • 71
    • 21
    • 14
    • 11
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This His Tag antibody is un-conjugated
    Application
    • 131
    • 84
    • 34
    • 25
    • 24
    • 15
    • 14
    • 14
    • 13
    • 13
    • 12
    • 7
    • 6
    • 6
    • 5
    • 4
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)
    Sequence
    HHHHHH
    Purification
    Affinity purification
    Immunogen
    A 6×His synthetic peptide (KLH-coupled).
    Isotype
    IgG
    Top Product
    Discover our top product His Tag Primary Antibody
  • Application Notes
    WB: 1:5000, IF: 1:2000, IP: 1:100
    Restrictions
    For Research Use only
  • Buffer
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid freeze / thaw cycles
    Storage
    -20 °C / -80 °C
  • Yang, Gu, Cheung, Yan, Tong, Jiang, Yang: "InsP3R-SEC5 interaction on phagosomes modulates innate immunity to Candida albicans by promoting cytosolic Ca2+ elevation and TBK1 activity." in: BMC biology, Vol. 16, Issue 1, pp. 46, (2018) (PubMed).

    Zhu, Yuan, Fan, Fan, Yang, Yin, Liu, Liu, Cao, Tian, Xue: "The synchronous TAG production with the growth by the expression of chloroplast transit peptide-fused ScPDAT in Chlamydomonas reinhardtii." in: Biotechnology for biofuels, Vol. 11, pp. 156, (2018) (PubMed).

    Liu, Xu, Zhu, Zhang, Chen, Zhou, Chen, Lin: "The calcium-dependent kinase OsCPK24 functions in cold stress responses in rice." in: Journal of integrative plant biology, Vol. 60, Issue 2, pp. 173-188, (2018) (PubMed).

    Liu, Li, Xie, Jia, Hou, Zou, Zhong: "Enhanced bacterial cellulose production by Gluconacetobacter xylinus via expression of Vitreoscilla hemoglobin and oxygen tension regulation." in: Applied microbiology and biotechnology, Vol. 102, Issue 3, pp. 1155-1165, (2018) (PubMed).

    Zhou, Zhao, Ni, Su, Wang, Xu: "Effects of environmental factors on C-type lectin recognition to zooxanthellae in the stony coral Pocillopora damicornis." in: Fish & shellfish immunology, Vol. 79, pp. 228-233, (2018) (PubMed).

    Jia, Wang, Zhang, Wang, Lv, Wang, Song: "Identification of a clip domain serine proteinase involved in immune defense in Chinese mitten crab Eriocheir sinensis." in: Fish & shellfish immunology, Vol. 74, pp. 332-340, (2018) (PubMed).

    Tao, Shi, Tang, Duszynski, Wang, Li, Suo, Tian, Liu, Suo et al.: "Transgenic Eimeria magna Pérard, 1925 Displays Similar Parasitological Properties to the Wild-type Strain and Induces an Exogenous Protein-Specific Immune Response in Rabbits (Oryctolagus cuniculus ..." in: Frontiers in immunology, Vol. 8, pp. 2, (2017) (PubMed).

    Wang, Li, Lu, Hu, Lin, Zeng, Zhou, Liu: "Molecular Characterization, Tissue Distribution and Expression, and Potential Antiviral Effects of TRIM32 in the Common Carp (Cyprinus carpio)." in: International journal of molecular sciences, Vol. 17, Issue 10, (2017) (PubMed).

    Jia, Zhang, Jiang, Wang, Wang, Song: "Comparative study of two single CRD C-type lectins, CgCLec-4 and CgCLec-5, from pacific oyster Crassostrea gigas." in: Fish & shellfish immunology, Vol. 59, pp. 220-232, (2017) (PubMed).

    Wang, Kuang, Lu, Lin, Liu: "Characterization and biological function analysis of the TRIM47 gene from common carp (Cyprinus carpio)." in: Gene, Vol. 627, pp. 188-193, (2017) (PubMed).

    Wang, Li, Xue, Cheng, Du: "Construction of a series of pCS2+ backbone-based Gateway vectors for overexpressing various tagged proteins in vertebrates." in: Acta biochimica et biophysica Sinica, Vol. 48, Issue 12, pp. 1128-1134, (2017) (PubMed).

    Zhang, Wang, Zhong, Luo, Shang, Liu, Chen, Fang, Xiao: "Ubiquitin-specific Protease 15 Negatively Regulates Virus-induced Type I Interferon Signaling via Catalytically-dependent and -independent Mechanisms." in: Scientific reports, Vol. 5, pp. 11220, (2016) (PubMed).

    Xu, Liu, Cai, Zhang, Xiong: "Heterologous expression and comparative characterization of vacuolar invertases from Cu-tolerant and non-tolerant populations of Elsholtzia haichowensis." in: Plant cell reports, Vol. 34, Issue 10, pp. 1781-90, (2016) (PubMed).

    Yan, Tian, Wang, Shao, Zheng, Zhang, Guo, Ding: "Pollen developmental defects in ZD-CMS rice line explored by cytological, molecular and proteomic approaches." in: Journal of proteomics, Vol. 108, pp. 110-23, (2015) (PubMed).

    Zhang, Li, Peng, Tang, Zhu, Qian, Xu, Qian: "Glycoprotein E2 of classical swine fever virus expressed by baculovirus induces the protective immune responses in rabbits." in: Vaccine, Vol. 32, Issue 49, pp. 6607-13, (2015) (PubMed).

  • Target
    His Tag
    Alternative Name
    His-Tag (His Tag Products)
    Target Type
    Tag
    Background
    Plasmid vectors for the expression of coding regions of eukaryotic genes in bacterial, insect and mammalian hosts are in common usage, such expression vectors are frequently used to encode hybrid fusion proteins consisting of a eukaryotic target protein and a specialized region designed to aid in the purification and visualization of the target protein. A system that has proven to be very successful relies on the insertion of a six histidine (His6) sequence in the N-terminus of the encoded protein, allowing for efficient coupling to Ni++- chelating resins and purification by single step affinity chromatography. This polyhistidine sequence can then be removed by specific cleavage at sites recognized by enzymes such as thrombin or enterokinase, permitting the separation of the target protein from the polyhistidine tag. Visualization of such fusion proteins can be achieved by utilizing antibodies generated against specific peptide sequences downstream from the multiple cloning site.       
You are here:
Support