KRT8 antibody

Catalog No. ABIN3024664

Product Details anti-KRT8 Antibody

Target: KRT8 (Keratin 8 (KRT8))

Reactivity: Human, Rat, Zebrafish (Danio rerio)

Host: Mouse

Clonality: Monoclonal

Conjugate: This KRT8 antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Protein G affinity chromatography

Immunogen: A cytoskeleton preparation containing Cytokeratin 8 (H1) and Keratin preparation from a human carcinoma (TS1) were used as the immunogen for this antibody cocktail.

Clone: H1-TS1

Isotype: IgG1

Application Details

Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate Buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,IHC (FFPE): 0.5-1.0 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the Cytokeratin 8 antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for KRT8

Target: KRT8 (Keratin 8 (KRT8))

Alternative Name: Cytokeratin 8 (KRT8 Products)

Synonyms: CARD2 antibody, CK-8 antibody, CK8 antibody, CYK8 antibody, K2C8 antibody, K8 antibody, KO antibody, AA960620 antibody, AL022697 antibody, AU019895 antibody, Card2 antibody, EndoA antibody, Krt-2.8 antibody, Krt2-8 antibody, CYKER antibody, KRT2-8 antibody, KERATIN8 antibody, ck8 antibody, cyk8 antibody, k2c8 antibody, card2 antibody, krt2-5 antibody, MGC69490 antibody, KRT8 antibody, DreK8 antibody, cb186 antibody, krt2-8 antibody, sb:cb186 antibody, wu:fa20h05 antibody, wu:fa95h10 antibody, wu:fb96c06 antibody, zf-K8 antibody, zfk8 antibody, DKFZp468F2127 antibody, keratin 8 antibody, keratin 8 S homeolog antibody, KRT8 antibody, Krt8 antibody, krt8.S antibody, krt8 antibody

Background: Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). Cytokeratin 8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibody that recognizes only cytokeratin 8 and 18. Cytokeratin 8 exists on several types of normal and neoplastic epithelia, including many ductal and glandular epithelia such as colon, stomach, small intestine, trachea, and esophagus as well as in transitional epithelium. antibody to Cytokeratin 8 does not react with skeletal muscle or nerve cells. Epithelioid sarcoma, chordoma, and adamantinoma show strong positivity corresponding to that of simple epithelia (with antibodies against Cytokeratin 8, 18 and 19). Reportedly, Cytokeratin 8 antibody is useful for the differentiation of lobular (""ring-like, perinuclear"") from ductal (""peripheral-predominant"") carcinoma of the breast.

Gene ID: 3856