TRPC6 antibody (Middle Region)

Catalog No. ABIN3029233

Product Details anti-TRPC6 Antibody

Target: TRPC6 (Transient Receptor Potential Cation Channel, Subfamily C, Member 6 (TRPC6))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRPC6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Antigen affinity

Immunogen: An amino acid sequence from the middle region of human TRPC6 (HDYFCKCNDCNQKQKHD) was used as the immunogen for this TRPC6 antibody.

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the TRPC6 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the TRPC6 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for TRPC6

Target: TRPC6 (Transient Receptor Potential Cation Channel, Subfamily C, Member 6 (TRPC6))

Alternative Name: TRPC6 (TRPC6 Products)

Synonyms: FSGS2 antibody, TRP6 antibody, AV025995 antibody, LLHWJM002 antibody, LLHWJM003 antibody, LLHWJM004 antibody, TRP-6 antibody, Trrp6 antibody, mtrp6 antibody, trp6 antibody, bZ1P14.9 antibody, si:rp71-1p14.9 antibody, trpc6 antibody, transient receptor potential cation channel subfamily C member 6 antibody, transient receptor potential cation channel, subfamily C, member 6 antibody, transient receptor potential cation channel, subfamily C, member 6a antibody, TRPC6 antibody, Trpc6 antibody, trpc6a antibody, trpc6 antibody

Background: Transient receptor potential cation channel, subfamily C, member 6, also known as TRP6, is a transient receptor potentialion channel. Northern blot analysis revealed that TRPC6 is expressed primarily in placenta, lung, spleen, ovary, and small intestine. The protein is a nonselective cation channel that is activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C. Although TRPC3, the closest structural relative of TRPC6, is activated in the same manner, human TRPC1 and mouse Trpc4 and Trpc5 were unresponsive to DAG. Immunofluorescence studies showed that most TRPC6 expression is confined to podocytes. It is also expressed in glomerular endothelial cells. Cardiac-specific overexpression of the protein in transgenic mice resulted in heightened sensitivity to stress, a propensity for lethal cardiac growth and heart failure, and an increase in Nfat-dependent expression of beta-myosin heavy chain(MYH7), a marker for pathologic hypertrophy. Studies of Trpc6 -/- mice showed that it has a role in regulation of smooth muscle tone in blood vessels and lung.

UniProt: Q9Y210