Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
Purification
Antigen affinity
Immunogen
An amino acid sequence from the N-terminus of human NPM1 (ELKADKDYHFKVDNDENEHQ) was used as the immunogen for this Nucleophosmin antibody (100% homologous in human, mouse and rat).
Variations in secondary/substrate sensitivities and test protocols may require the Nucleophosmin antibody to be titrated for optimal performance.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL,Immunocytochemistry: 0.5-1 μg/mL
Restrictions
For Research Use only
Buffer
0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
Storage
-20 °C
Storage Comment
The lyophilized Nucleophosmin antibody can be stored at 4°C to -20°C. After reconstitution, aliquot and store at -20°C. Avoid repeated freeze/thaws.
Nucleophosmin, also known as NPM, is a protein that in humans is encoded by the NPM1 gene. The gene maps to chromosome 5q35. Nucleophosmin is likely involved in the assembly of ribosomal proteins into ribosomes. An electron microscopic study indicated that Nucleophosmin is concentrated in the granular region of the nucleolus, where ribosome assembly occurs.