GRIN1/NMDAR1 antibody (N-Term)

Catalog No. ABIN3044347

Product Details anti-GRIN1/NMDAR1 Antibody

Target: GRIN1/NMDAR1 (GRIN1) (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 1 (GRIN1))

Binding Specificity: AA 36-53, N-Term

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRIN1/NMDAR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Glutamate receptor ionotropic, NMDA 1(GRIN1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequence: RKHEQMFREA VNQANKRH

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Glutamate receptor ionotropic, NMDA 1(GRIN1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: glutamate receptor, ionotropic, N-methyl D-aspartate 1
Protein Name: Glutamate receptor ionotropic, NMDA 1

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human NMDAR1(36-53aa RKHEQMFREAVNQANKRH), identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-GRIN1/NMDAR1 antibody (ABIN3044347)

Feng, Du: "Mechanisms responsible for the effect of median nerve electrical stimulation on traumatic brain injury-induced coma: orexin-A-mediated N-methyl-D-aspartate receptor subunit NR1 upregulation." in: Neural regeneration research, Vol. 11, Issue 6, pp. 951-6, (2016) (PubMed).

Wang, Wang, Fu, Zhang, Wu, Guo, Wang: "Expression of N-methyl-d-aspartate receptor 1 and its phosphorylated state in basal ganglia of a neonatal piglet hypoxic-ischemic brain injury model: a controlled study of (1)H MRS." in: European journal of paediatric neurology : EJPN : official journal of the European Paediatric Neurology Society, Vol. 16, Issue 5, pp. 492-500, (2012) (PubMed).

Shang, Luo, Deng, Wang, Huang, Feng, Yue: "Expression of N-methyl-D-aspartate receptor and its effect on nitric oxide production of rat alveolar macrophages." in: Nitric oxide : biology and chemistry / official journal of the Nitric Oxide Society, Vol. 23, Issue 4, pp. 327-31, (2010) (PubMed).

Target Details for GRIN1/NMDAR1

Target: GRIN1/NMDAR1 (GRIN1) (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 1 (GRIN1))

Alternative Name: GRIN1 (GRIN1 Products)

Synonyms: GluN1 antibody, MRD8 antibody, NMDA1 antibody, NMDAR1 antibody, NR1 antibody, nmda1 antibody, nmdar1 antibody, GB19253 antibody, GRIN1 antibody, NMD-R1 antibody, Z16 antibody, GluRdelta1 antibody, GluRzeta1 antibody, M100174 antibody, Nmdar antibody, Rgsc174 antibody, NMDAR1.1 antibody, zgc:152678 antibody, glutamate ionotropic receptor NMDA type subunit 1 antibody, glutamate receptor, ionotropic, N-methyl D-aspartate 1 S homeolog antibody, NMDA receptor 1 antibody, G protein-regulated inducer of neurite outgrowth 1 antibody, glutamate receptor, ionotropic, NMDA1 (zeta 1) antibody, glutamate receptor, ionotropic, N-methyl D-aspartate 1a antibody, GRIN1 antibody, grin1.S antibody, grin1 antibody, Grin1 antibody, Nmdar1 antibody, Gprin1 antibody, grin1a antibody

Background: The NMDA receptor(NMDAR) is a specific type of ionotropic glutamate receptor. NMDA(N-methyl D-aspartate) is the name of a selective agonist that binds to NMDA receptors but not to other glutamate receptors. Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. NMDAR1 gene is mapped to 9q34.3 and encodes a 938-amino acid protein which showed high evolutionary conservation in structure and physiologic properties. It consists of 21 exons distributed over about 31 kb. Three of the exons that are alternatively spliced in the rat and which produce 8 isoforms in that species were also present in the human sequence. The promoter region contained 2 DNA binding sites for the homeobox proteins 'even-skipped'. The gene is a candidate for the site of the mutation in torsion dystonia.The NMDA receptor is a non-specific cation channel and thus directly contributes to excitatory synaptic transmission by depolarizing the postsynaptic cell. NMDA receptors are modulated by a number of endogenous and exogenous compounds and play a key role in a wide range of physiologic and pathologic processes, such as excitotoxicity.

Synonyms: GluN1 antibody|Glutamate[NMDA] receptor subunit zeta 1 antibody|Glutamate[NMDA] receptor subunit zeta-1 antibody|Glutamate receptor ionotropic N methyl D aspartate 1 antibody|Grin 1 antibody|Grin1 antibody|MRD8 antibody|N methyl D aspartate receptor antibody|N methyl D aspartate receptor channel antibody|N methyl D aspartate receptor channel subunit zeta 1 antibody|N methyl D aspartate receptor subunit NR1 antibody|N-methyl-D-aspartate receptor antibody|N-methyl-D-aspartate receptor subunit NR1 antibody|NMD-R1 antibody|NMDA 1 antibody|NMDA NR1 antibody|NMDA R1 antibody|NMDA receptor 1 antibody|NMDA1 antibody|NMDAR 1 antibody|NMDAR antibody|Nmdar1 antibody|NMDZ1_HUMAN antibody|NR 1 antibody|NR1 antibody

UniProt: Q05586

Pathways: Synaptic Membrane, Feeding Behaviour, Regulation of long-term Neuronal Synaptic Plasticity