Activin Receptor-Like Kinase 2,Activin Receptor-Like Kinase 2 is a member of the superfamily of type I receptors for transforming growth factor- (TGF- ). This receptor binds both activin and bone morphogenic proteins (BMPs). In mice, these receptors are expressed mainly in the extraembryonic visceral endoderm before gastrulation and later in both embryonic and extraembryonic cells during gastrulation. Also, it has been shown that ALK2 associates with and phosphorylates Smad1 and also regulated Smad5 and Smad8 phosphorylation. Additionally, it induces the Tlx2 promoter in the absense of BMP7.
Purification
Ammonium Sulfate Precipitation
Immunogen
Recombinant protein derived from the extracellular domain of the chicken ALK2 protein. Protein shares significant homology with the mouse, rat and human sequences.
Antibody can be used for Western blotting (5-10 μg/mL), immunoprecipitation (5 μg/mg of protein lysate and immunohistochemistry on paraffin embedded tissue sections (5-10 μg/mL). Optimal concentration should be evaluated by serial dilutions.
Restrictions
For Research Use only
Buffer
Provided as solution in phosphate buffered saline with 0.08 % sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles