ALOX5 antibody (pSer523)

Catalog No. ABIN318947

Product Details anti-ALOX5 Antibody

Target: ALOX5 (Arachidonate 5-Lipoxygenase (ALOX5))

Binding Specificity: pSer523

Reactivity: Human, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALOX5 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: This antibody specifically recognises human 5-lipoxygenase (5-LO, also known as arachidonate 5-lipoxygenase) when phosphorylated at Ser523.

Purification: Purified

Immunogen: Synthetic phosphopeptide corresponding to an amino acid sequence within human 5-lipoxygenase which includes phosphorylated Ser523.

Isotype: IgG

Application Details

Application Notes: Western Blot: 1: 1000, detects a doublet of approximately 80 kDa in rat cortex lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES pH 7.5, 150 mM NaCl containing 0.09 % Sodium Azide, 0.01 % Bovine Serum Albumin, 50 % Glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ALOX5

Target: ALOX5 (Arachidonate 5-Lipoxygenase (ALOX5))

Alternative Name: ALOX5 / LOG5 (ALOX5 Products)

Synonyms: 5-LO antibody, 5-LOX antibody, 5LPG antibody, LOG5 antibody, 5LO antibody, 5LX antibody, AI850497 antibody, F730011J02 antibody, LOX5A antibody, si:dkey-194n13.2 antibody, lox5 antibody, arachidonate 5-lipoxygenase antibody, arachidonate 5-lipoxygenase a antibody, Arachidonate 5-lipoxygenase antibody, ALOX5 antibody, Alox5 antibody, alox5a antibody, AFUA_4G02770 antibody, AOR_1_1362014 antibody, lox5 antibody

Background: 5-LO is an enzyme that catalyses the synthesis of leukotrienes (LT's) from arachidonic acid (a polyunsaturated fatty acid), by the insertion of an oxygen into the arachidonic backbone. 5-LO requires calcium, ATP and the 5LOactivating protein as cofactors for full activity. Leukotrienes are synthesised and secreted by leukocytes in response to immunologic or inflammatory stimuli, such as in asthma and allergic reactions. The biological effects of LT's include leukocyte chemotaxis, pulmonary airway constriction, mucous hypersecretion, vasoconstriction and increased vascular permeability. I recent years 5-LO has also been implicated in the regulation of cell proliferation and apoptosis and has been found in a broad variety of cancer cells.Synonyms: 5-LO, 5-lipoxygenase, Arachidonate 5-lipoxygenase

Gene ID: 240

NCBI Accession: NP_000689

UniProt: P09917