TH
Reactivity: Rat
WB, IHC, IF
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Immunohistochemistry on frozen sections: 1: 1000. Western Blot: 1: 1000,detects a band of approximately 60 kDa in PC-12 cell lysates,following stimulation by Okadaic acid. Immunofluorescence: 1: 1000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Buffer
10 mM HEPES, pH 7.5 containing 0.09 % Sodium Azide, 0.01 % Bovine Serum Albumin and 50 % Glycerol
Preservative
Sodium azide
Precaution of Use
This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Tyrosine hydroxylase (TH) catalyzes the rate-limiting step in the biosynthetic pathway of the catecholamines dopamine (DA), norepinephrine, and epinephrine. The enzyme exists as a tetramer, with each subunit composed of an N-terminal regulatory domain and a C-terminal catalytic domain. Phosphorylation of TH has been shown to occur at several serine residues. Phosphorylation at serine 40 results in an increase in hydroxylase activity, and phosphorylation at serine 19 is reported to promote phosphorylation of the serine 40 residue. Tyrosine hydroxylase is regularly used as a marker for dopaminergic neurons, which is particularly relevant for research into ParkinsonSynonyms: TYH, Tyrosine 3-hydroxylase