eIF4EBP1 antibody

Catalog No. ABIN3209983

The Mouse Monoclonal anti-eIF4EBP1 antibody is suitable to detect eIF4EBP1 in samples from Human. It has been validated for WB, IHC and ELISA.

Quick Overview for eIF4EBP1 antibody (ABIN3209983)

Target: eIF4EBP1 (EIF4EBP1) (Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This eIF4EBP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: 11G12C11

Product Details anti-eIF4EBP1 Antibody

Purification: Purified antibody.

Immunogen: 4 EBP1 antibody was raised in Mouse using a purified recombinant fragment of 4 BP1 expressed in E. coli as the immunogen.

Isotype: IgG1

Application Details

Application Notes: ELISA: 1:10,000, IHC: 1:200-1:1000, WB: 1:500-1:2000
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: Lot specific

Buffer: Purified antibody supplied in PBS containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C for short term storage. Store at -20 °C for long term storage.

Target Details for eIF4EBP1

Target: eIF4EBP1 (EIF4EBP1) (Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1))

Alternative Name: 4EBP1

Background: 4EBP1 which is located on chromosome 8p12, with 118-amino acid protein (about 13kDa). Binding of eIF4EBP1 to eIF4E is reversible and is dependent on the phosphorylation status of eIF4EBP1. Non phosphorylated eIF4EBP1 will bind strongly to eIF4E while the phosphorylated form will not. Akt, TOR, MAP kinase, S6 kinase, and Cdc2 are known kinases capable of inactivating eIF4EBP1 binding to eIF4E by phosphorylating either threonines 35, 45, 69 or serine 64. 4EBP1 functions as a translation repressor. Synonyms: Monoclonal 4EBP1 antibody, Anti-4EBP1 antibody, 4E-BP1 antibody, 4E BP1 antibody, Eukaryotic translation initiation factor 4E-binding protein 1 antibody, BP-1 antibody, MGC4316 antibody.

Pathways: MAPK Signaling, PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Regulation of Cell Size, BCR Signaling