CMA1 antibody (AA 20-247)
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- Target See all CMA1 Antibodies
- CMA1 (Chymase 1, Mast Cell (CMA1))
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Binding Specificity
- AA 20-247
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CMA1 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunoprecipitation (IP)
- Specificity
- Recognizes human Chymase at ~30kD. No cross reactivity with r mMCPT-1, r mMCPT-6, r mMCPT-7 or r mMCPT-11.
- Purification
- Protein G purified
- Immunogen
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NS0-derived rhChymase. (aa 20 - 247).
Type of Immunogen: Recombinant protein - Isotype
- IgG2a
- Top Product
- Discover our top product CMA1 Primary Antibody
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- Application Notes
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Approved: ELISA (0.5 - 1 μg/mL), IP (25 μg/mL), WB (1 - 2 μg/mL)
Usage: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Direct ELISA: 0.5-1 μg/mL. Detection limit for rhChymase is ~1 ng/well. Western Blot: 1-2 μg/mL. Detection limit for rhChymase is. ~10 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Immunoprecipitation: 25 μg/mL from conditioned cell culture medium. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- 200 μL sterile PBS
- Concentration
- Lot specific
- Buffer
- Lyophilized from PBS, 5 % trehalose.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C,-20 °C
- Storage Comment
- 4°C or -20°C, Avoid freeze-thaw cycles.
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- Target
- CMA1 (Chymase 1, Mast Cell (CMA1))
- Alternative Name
- CMA1 / MCT1 / Chymase (CMA1 Products)
- Synonyms
- CYH antibody, MCT1 antibody, chymase antibody, MMCP-5 antibody, Mcp-5 antibody, Mcp5 antibody, Mcpt5 antibody, MCP3P antibody, CMA1 antibody, Chymase antibody, chymase 1 antibody, chymase 1, mast cell antibody, chymase-like antibody, CMA1 antibody, Cma1 antibody, LOC100008644 antibody
- Background
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Name/Gene ID: CMA1
Subfamily: Serine S1
Family: Protease
Synonyms: CMA1, Chymase, Chymase, heart, Chymase, mast cell, CYH, Mast cell chymase, Mcpt5, MCT1, Mast cell protease 5, Mast cell protease I, Heart chymase, Alpha-chymase, Chymase 1, Chymase 1, mast cell, CYM - Gene ID
- 1215
- UniProt
- P23946
- Pathways
- ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Carbohydrate Homeostasis
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