Tyrosine Hydroxylase antibody (pSer40)
-
- Target See all Tyrosine Hydroxylase (TH) Antibodies
- Tyrosine Hydroxylase (TH)
-
Binding Specificity
- pSer40
-
Reactivity
- Human, Rat
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- This Tyrosine Hydroxylase antibody is un-conjugated
-
Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Brand
- IHC-plus™
- Specificity
- Specific for the ~60k tyrosine hydroxylase protein phosphorylated at Ser40. Some higher molecular weight bands may be detected by the antibody depending upon the brain region being studied, protein loads and the detection methods used. The antibody has three orders of magnitude selectivity over dephospho TH.
- Purification
- Immunoaffinity purified
- Immunogen
- Phosphopeptide corresponding to amino acid residues surrounding the phosphoSer40 of rat tyrosine hydroxylase.
- Top Product
- Discover our top product TH Primary Antibody
-
-
- Application Notes
-
Approved: IF (1:1000), IHC, IHC-Fr (1:1000), IHC-P (5 μg/mL), WB (1:1000)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. - Comment
-
Target Species of Antibody: Rat
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 10 mM HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol.
- Storage
- -20 °C,-80 °C
- Storage Comment
-
Short term: -20°C
Long term: -80°C.
-
- Target
- Tyrosine Hydroxylase (TH)
- Alternative Name
- TH / Tyrosine Hydroxylase (TH Products)
- Synonyms
- DYT14 antibody, DYT5b antibody, TYH antibody, The antibody, CG10118 antibody, DH65B antibody, DTH antibody, Dmel\\CG10118 antibody, Pale antibody, Ple antibody, TH antibody, Th antibody, VII antibody, dTH1 antibody, dTH65B antibody, pale/ple antibody, th antibody, dyt14 antibody, dyt5b antibody, tyh antibody, tyrosine hydroxylase antibody, pale antibody, tyrosine 3-monooxygenase antibody, tyrosine hydroxylase S homeolog antibody, TH antibody, Th antibody, ple antibody, th antibody, th.S antibody, LOC100008895 antibody
- Background
-
Name/Gene ID: TH
Family: Monooxygenase
Synonyms: TH, DYT14, Dystonia 14, TYH, Tyrosine 3-hydroxylase, Tyrosine hydroxylase, DYT5b, Tyrosine 3-monooxygenase - Gene ID
- 7054
- UniProt
- P07101
- Pathways
- Dopaminergic Neurogenesis, Response to Water Deprivation, Sensory Perception of Sound, Carbohydrate Homeostasis, Feeding Behaviour
-