MIF antibody (AA 103-115)
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- Target See all MIF Antibodies
- MIF (Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF))
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Binding Specificity
- AA 103-115
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Reactivity
- Human, Monkey
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This MIF antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Brand
- IHC-plus™
- Specificity
- Human MIF.
- Predicted Reactivity
- Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%) Mouse, Rat, Sheep, Elephant, Bovine, Horse, Rabbit, Pig, Turkey, Chicken, Platypus, Xenopus (92%) Hamster (85%).
- Purification
- Immunoaffinity purified
- Immunogen
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Synthetic peptide C-NAANVGWNNSTFA from the C-terminus of human MIF (NP_002406.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%), Mouse, Rat, Sheep, Elephant, Bovine, Horse, Rabbit, Pig, Turkey, Chicken, Platypus, Xenopus (92%), Hamster (85%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product MIF Primary Antibody
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- Application Notes
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Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:128000), WB (0.01 - 0.03 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Tris-buffered saline, pH 7.3, 0.5 % BSA, 0.02 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Store at -20°C. Avoid freeze-thaw cycles.
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- Target
- MIF (Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF))
- Alternative Name
- MIF (MIF Products)
- Synonyms
- mif antibody, Mif antibody, gif antibody, glif antibody, mmif antibody, LOC100136498 antibody, LOC100284350 antibody, LOC100284546 antibody, GIF antibody, Glif antibody, GLIF antibody, MMIF antibody, macrophage migration inhibitory factor L homeolog antibody, macrophage migration inhibitory factor antibody, macrophage migration inhibitory factor (glycosylation-inhibiting factor) antibody, mif.L antibody, mif antibody, MIF antibody, Mif antibody, PHATRDRAFT_49660 antibody, LOC100136498 antibody, cl405_1 antibody, LOC100284546 antibody
- Background
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Name/Gene ID: MIF
Synonyms: MIF, GLIF, L-dopachrome tautomerase, L-dopachrome isomerase, MMIF, Phenylpyruvate tautomerase - Gene ID
- 4282
- NCBI Accession
- NP_002406
- UniProt
- P14174
- Pathways
- Regulation of Systemic Arterial Blood Pressure by Hormones, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling
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